Fibers of cellulose sugarcane bagasse with bromelain enzyme immobilized to application in dressing

Author(s)

Costa, Silgia A. ^[1] ; Ceron, Annie A. ^[1] ; Petreca, Bruna B. ^[2] ; Costa, Sirlene M. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Arts Sci & Humanities Text & Fash Course, Av Arlindo Betio 1000, Parque Ecol Tiete, BR-03828080 Sao Paulo, SP - Brazil

^[2] Mat Sci Royal Coll Art, London SW7 2EU - England

• Document type: Journal article
• Source: SN APPLIED SCIENCES; v. 2, n. 2 FEB 2020.
• Web of Science Citations: 0

Abstract

The enzymes have been gaining more importance in different fields, among the most important the pharmaceutical field. However, due to the protein nature of the enzymes, a significant part of them presents high instability under certain conditions of use. A great way to stabilize them is to use immobilization techniques. Bromelain from pineapple has shown potential to be used in the treatment of burn-like skin injuries and superficial injuries. Biocatalytic textile fibers from cellulose of sugarcane bagasse were prepared using surface functionalization with epichlorohydrin (4% v/v) and glutaraldehyde (0.5% v/v), and 1-ethyl-(3-dimethylaminopropyl) carbodiimide and bromelain immobilization by covalent bonding in the fibers. The best immobilization results for the bromelain enzyme immobilization were using the aminopropyltriethoxysilane and glutaraldehyde activating agent at pH 7 to values of 68.97% and 88.14% for total protein content and enzyme activity, respectively. Considering the approach described in this paper, others advanced materials from pulp fibers and bioprocesses might be developed using bromelain and other enzymes for the target applications. (AU)

• FAPESP's process: 06/56029-3 - Development of textile fibers from sugarcane bagasse cellulose with the incorporation of medicines and enzymes for medical applications
• Grantee: Sirlene Maria da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral