Immediate and Late Effects of Early Weaning on Rat Gastric Cell Differentiation

Author(s)

Teles Silva, Melissa ^[1] ; Mesquita da Silva, Kethleen ^[1] ; Campos Rattes, Isadora ^[1] ; Maria Agostini Zonta, Gizela ^[1] ; Vasques da Costa, Aline ^[1] ; Galvao Figueredo Costa, Raquel ^[1] ; Karen Cordeiro Nogueira, Ludimila ^[1] ; Ogias, Daniela ^[1] ; Gama, Patricia ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 1 JAN 1 2020.
• Web of Science Citations: 0

Abstract

Background: Gastric glands grow and cells reach differentiation at weaning in rats. By considering that early weaning (EW) can affect the timing of development, we aimed to compare molecular and cellular markers of differentiation in pups and adults. Methods: Wistar rats were separated into suckling-control (S) and EW groups at 15 days. Stomachs were collected at 15, 18, and 60 days for RNA and protein extraction, and morphology. Results: After EW, the expression of genes involved in differentiation (Atp4b, Bhlha15 and Pgc) augmented (18 days), and Atp4b and Gif were high at 60 days. EW increased the number of zymogenic cells (ZC) in pups and adults and augmented mucous neck cells only at 18 days, whereas parietal and transition cells (TC) were unchanged. Conclusions: EW affected the gastric mucosa mostly in a transient manner as the changes in gene expression and distribution of differentiated cells that were detected in pups were not fully maintained in adults, except for the size of ZC population. We concluded that though most of EW effects were immediate, such nutritional change in the infancy might affect part of gastric digestive functions in a permanent manner, as some markers were kept unbalanced in the adulthood. (AU)

• FAPESP's process: 11/17415-3 - Postnatal development of gastric mucosa: interaction among feeding, hormones and growth factors
• Grantee: Patrícia Gama
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/10948-9 - Neonatal maternal separation impacts on gastric mucosa development in pups and adult rats: Role of corticosterone and GR
• Grantee: Daniela Ogias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/21449-9 - Gene expression, cell differentiation and gastric functions: can alimentary changes during postnatal development disturb the growth program in rats?
• Grantee: Patrícia Gama
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/21944-5 - Identification of the stem cell niche in the gastric mucosa of developing rats
• Grantee: Isadora Campos Rattes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/24972-5 - Effects of early weaning on molecular markers during postnatal growth and development of intestinal
• Grantee: Raquel Galvão Figuerêdo Costa
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 18/07782-8 - Effects of early weaning on molecular markers during postnatal growth and development of intestinal mucosa in the rat.
• Grantee: Patrícia Gama
• Support Opportunities: Regular Research Grants