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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of embryonic origin on osteogenic potential and bone repair capacity of rat calvarial osteoblasts

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Author(s):
Souza, Alann Thaffarell Portilho [1] ; Lopes, Helena Bacha [1] ; Freitas, Gileade Pereira [1] ; Ferraz, Emanuela Prado [2] ; Oliveira, Fabiola Singaretti [1] ; Almeida, Adriana Luisa Goncalves [1] ; Weffort, Denise [1] ; Beloti, Marcio Mateus [1] ; Rosa, Adalberto Luiz [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Bone Res Lab, Sch Dent Ribeirao Preto, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Maxillofacial Surg Prosthesis & Traumatol, Sch Dent, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF BONE AND MINERAL METABOLISM; v. 38, n. 4 FEB 2020.
Web of Science Citations: 0
Abstract

Introduction The aim of this study was to evaluate the in vitro osteogenic potential of osteoblasts from neural crest-derived frontal bone (OB-NC) and mesoderm-derived parietal bone (OB-MS) and the bone formation induced by them when injected into calvarial defects. Materials and methods Calvarial bones were collected from newborn Wistar rats (3-day old) and characterized as frontal and parietal prior to OB-NC and OB-MS harvesting. The cells were cultured, and several parameters of osteoblast differentiation were evaluated. These cells, or PBS without cells (control), were locally injected into 5-mm rat calvarial defects (5 x 10(6) cells/defect) and after 4 weeks bone formation was evaluated by morphometric and histological analyses. Results The characterization of frontal and parietal bones assured the different embryonic origin of both cell populations, OB-NC and OB-MS. The OB-NC presented higher proliferation while the OB-MS presented higher alkaline phosphatase (ALP) activity, extracellular matrix mineralization and gene expression of runt-related transcription factor 2, Alp, bone sialoprotein and osteocalcin revealing their high osteogenic potential. mu CT analysis indicated that there was higher amount of bone formation in defects injected with both OB-NC and OB-MS compared to the control. Moreover, the bone tissue formed by both cells displayed the same histological characteristics. Conclusions Despite the distinct in vitro osteogenic potential, OB-NC and OB-MS induced similar bone repair in a rat calvarial defect model. Thus, osteoblasts, irrespective of their in vitro osteogenic potential linked to embryonic origins, seem to be suitable for cell-based therapies aiming to repair bone defects. (AU)

FAPESP's process: 15/21439-6 - Mesenchymal stem cells and osteoblasts: evaluation of molecular signatures and their potential for bone regeneration
Grantee:Adalberto Luiz Rosa
Support type: Regular Research Grants
FAPESP's process: 09/54142-5 - Creation of a Multi-User Laboratory for the analysis of three-dimensional images of biological tissues and biomaterials (3D BIOLAB)
Grantee:Adalberto Luiz Rosa
Support type: Multi-user Equipment Program