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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Silencing of nuclear factor kappa b 1 gene expression inhibits colony formation, cell migration and invasion via the downregulation of interleukin 1 beta and matrix metallopeptidase 9 in renal cell carcinoma

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Author(s):
Teixeira, Luiz Felipe S. [1] ; Peron, Jean Pierre S. [2] ; Bellini, Maria Helena [1]
Total Authors: 3
Affiliation:
[1] IPEN CNEN SP, Dept Biotechnol, 2242 Ave Lineu Prestes, Cidade Univ, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Immunol, ICB 4, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MOLECULAR BIOLOGY REPORTS; v. 47, n. 2, p. 1143-1151, FEB 2020.
Web of Science Citations: 0
Abstract

Renal cell carcinoma (RCC) is a highly deadly urological tumor due to its high metastatic incidence and its notorious chemoresistance. The nuclear transcription factor kappa B (NF-kappa B) family has been associated with apoptosis resistance and cellular invasion in RCC. The purpose of this study was to evaluate the impact of NF-kappa B1 gene silencing on the colony formation, cell migration and invasion abilities of the RCC cell line. Renca-mock and Renca-shRNA-NF-kappa B1 cells were used in this work. NF-kappa B1 downregulation was assessed by western blotting. The mRNA expression levels of interleukin-1 beta (IL-1 beta) and MMP-9 were assessed by real-time quantitative polymerase chain reaction (RT-qPCR). The IL-1 beta levels in the culture media were determined by a commercial ELISA kit. The MMP-9 protein expression and gelatinolytic activity were evaluated by western blotting and zymography, respectively, and the migration and invasion abilities were analysed. The expression levels of p105 and p50 in Renca-shRNA-NF-kappa Bmoc1 cells were significantly reduced compared with those in the Renca-mock cells. The colony numbers of shRNA-NF-kB1 cells were lower than the colony numbers of the Renca-mock cells. NF-kappa B1 knockdown inhibited the cell migration and invasion of Renca-shRNA-NF-kappa B1 cells. These cells also exhibited reduced levels of IL-1 beta. The MMP-9 expression and activity levels were significantly reduced in Renca-shRNA-NF-kappa B1 cells. Taken together, these results indicate that the downregulation of NF-kappa B1 suppresses the tumourigenicity of RCC by reducing MMP-9 expression and activity; thus, NF-kappa B1 could be a molecular target for RCC treatment. (AU)

FAPESP's process: 14/19265-7 - Functional assessment of NF-Kb1 gene in the tumorigenesis of renal cell carcinoma cell lines
Grantee:Maria Helena Bellini Marumo
Support Opportunities: Regular Research Grants