| Full text | |
| Author(s): |
Alves Camelo, Lidia Cristina
[1]
;
Dias Santos, Gabriela de Souza
[1]
;
de Souza, Ranyere Lucena
[1, 2]
;
Faria Soares, Cleide Mara
[1, 2]
;
Brandao Pereira, Jorge Fernando
[3]
;
Lima, Alvaro Silva
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Tiradentes, Programa Posgrad Engn Proc, Av Murilo Dantas 300, BR-49032490 Aracaju, Sergipe - Brazil
[2] Inst Tecnol & Pesquisa, Av Murilo Dantas 300, BR-49032490 Aracaju, Sergipe - Brazil
[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Rodovia Araraquara Jau Km 01, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Fluid Phase Equilibria; v. 507, MAR 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The aim of this study was to evaluate the formation of aqueous two-phase systems (ATPS) based on different protic ionic liquids (PIL), synthesized using amines, and organic or inorganic acid, and acetonitrile (ACN). The ATPSs are used to pre-purify genipin from genipap (Genipa americana L.). All PILs (purity above 98%) were able to form ATPSs with ACN, the size of the biphasic region being directly dependent on salting-out aptitude of PIL anions and relative hydrophilicity of the corresponding cations. The highest solid liquid extraction of genipin from genipap was achieved by using aqueous ACN solution at 75% (16.79 +/- 0.06 mg g(-1) dry raw material) as extractant. After the extraction stage, the ATPS was used as an integrated platform to pre-purify the genipin present in the extract. Three different overall mixture point compositions were tested, and those formed by {[}MEA]{[}H(2)Cit] (25 wt%) + ACN (45 wt%) + water (30 wt%) at 298.15 +/- 1.00 K and 0.10 +/- 0.01 MPa showed the highest selectivity (S = 4.202 +/- 0.009) and recovery of genipin in top phase (R-TG = 56.74 +/- 0.04%), and the purification factor in top phase was 5.9 fold. (C) 2019 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase) |
| Grantee: | Jorge Pereira |
| Support Opportunities: | Research Grants - Young Investigators Grants |