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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evolutionary perspectives on bee mtDNA from mito-OMICS analyses of a solitary species

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Author(s):
Francoso, Elaine [1] ; de Souza Araujo, Natalia [1, 2] ; Ricardo, Paulo Cseri [1] ; Santos, Priscila Karla Ferreira [1] ; Zuntini, Alexandre Rizzo [3] ; Arias, Maria Cristina [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Rua Matao 277, Sala 320, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Libre Bruxelles, Dept Evolutionary Biol & Ecol, Ave FD Roosevelt 50, B-1050 Brussels - Belgium
[3] Royal Bot Gardens, Richmond TW9 3AE, Surrey - England
Total Affiliations: 3
Document type: Journal article
Source: Apidologie; v. 51, n. 4 FEB 2020.
Web of Science Citations: 1
Abstract

The analysis of mitochondrial DNA polymorphism has been applied in multiple organisms to obtain information about species biology, ecology, population dynamics, and evolution. In this manuscript, the complete sequencing and characterization of the mitochondrial genome (mtGenome) of Tetrapedia diversipes are reported and discussed from comparative and evolutionary perspectives among all mtGenomes available for bees so far. The T. diversipes mtGenome is 15,358 bp long and exhibits the typical set of genes and an A+T-rich region of 588 bp. The overall base composition is biased towards A/T (84.3%), with 42.6% A, 41.7% T, 9.8% C, and 5.9% G nucleotides. The obtained data also comprise the mitochondrial DNA methylation and single-nucleotide polymorphic sites of one T. diversipes population. Transcription follows the ``tRNA punctuation{''} model, with at least three primary polycistronic transcripts that are posteriorly processed. Additionally, higher expression rates of the 16S gene suggest the existence of an exclusive transcription site in this region, and the differential expression of the 12S gene between larvae and adults reveals different isoforms for this gene. The sequence order of protein-coding and rRNA genes is conserved across different bee lineages, and differences are restricted to tRNA gene positions. The present results characterize numerous understudied aspects of bee mtGenomes, and a major evolutionary review of this molecule within the group is provided. Therefore, this work is a valuable resource for studying mitochondrial molecular biology and evolution in bees. (AU)

FAPESP's process: 12/18531-0 - Expression of Genes Involved in Social Behavior in Bees with Different Levels of Eusociality
Grantee:Natália de Souza Araujo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/20548-2 - Phylogeography of B. morio and B. pauloensis (Hymenoptera: Apidae)
Grantee:Elaine Aparecida Françoso
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 09/07124-1 - Phylogeography of B. morio and B. atratus (Hymenoptera: Apidae)
Grantee:Elaine Aparecida Françoso
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/03961-1 - Comparative phylogeography and conservation of Bombus morio e Bombus pauloensis (Hymenoptera: Apidae)
Grantee:Elaine Aparecida Françoso
Support type: Scholarships abroad - Research Internship - Doctorate (Direct)
FAPESP's process: 13/12530-4 - Population, evolutionary and genomic studies in bees
Grantee:Maria Cristina Arias
Support type: Regular Research Grants