Use of Algae Biomass Obtained by Single-Step Mild Acid Hydrolysis in Hydrogen Production by the beta-Glucosidase-Producing Clostridium beijerinckii Br21

Author(s)

Fonseca, Bruna Constante ^[1] ; Dalbelo, Giovanni ^[1] ; Gelli, Valeria Cress ^[2] ; Carli, Sibeli ^[1] ; Meleiro, Luana Parras ^[1] ; Zimbardi, Ana Lucia Ribeiro Latorre ^[1] ; Furriel, Rosa Prazeres Melo ^[1] ; Tapia, Delia Rita ^[1] ; Reginatto, Valeria ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ave Bandeirantes 3900, BR-14040030 Ribeirao Preto, SP - Brazil

^[2] Agencia Paulista Pesquisa Agr, Secretaria Agr & Abastecimento Estado Sao Paulo, Nucleo Pesquisa & Desenvolvimento Litoral Norte, Dept Pesca, Ubatuba, SP - Brazil

• Document type: Journal article
• Source: WASTE AND BIOMASS VALORIZATION; v. 11, n. 4, p. 1393-1402, APR 2020.
• Web of Science Citations: 1

Abstract

Macroalgae biomass is a potential feedstock for fermentative H-2 production: it has high carbohydrate concentration and is lignin-free. Here, we optimize a mild acid treatment of Kappaphycus alvarezii biomass by an experimental design 2(4). The optimal acid treatment conditions were 90 degrees C, HCl concentration of 55.9 mmol/L, 0.375 g of algae mass, and 8 h of treatment. Under these conditions, the hydrolysate presented mono-, but also di- and oligosaccharides. We used this hydrolysate as substrate for fermentative hydrogen (H-2) production by the Clostridium beijerinckii Br21 grown in two different conditions: (1) in medium containing galactose and no beta-glucosidase activity; (2) in medium with cellobiose to stimulate beta-glucosidase activity, which was 0.33U/mL. The fermentative assay conducted in the presence of the C. beijerinckii Br21 with beta-glucosidase activity provided higher H-2 concentration and yield as compared to the assay accomplished in the presence of the inoculum with no beta-glucosidase activity-171.76 +/- 2.10 and 140.95 +/- 10.92 mL of H-2/L, and 70.3 +/- 0.9 and 62.7 +/- 4.9 mL of H-2/g of dry algae, respectively. Therefore, a fermentative beta-glucosidase-producing organism such as C. beijerinckii Br21 can potentially complete biomass saccharification for later renewable H-2 production. (AU)

• FAPESP's process: 15/06074-1 - Third-generation biohydrogen production from algae biomass and its hydrolysis derivatives
• Grantee: Valeria Reginatto Spiller
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants