Synergistic potential of 1 alpha,25-dihydroxyvitamin D3 and calcium-aluminate-chitosan scaffolds with dental pulp cells

Author(s):Show less -

Bordini, Ester Alves Ferreira ^[1] ; Cassiano, Fernanda Balestrero ^[1] ; Silva, Isabela Sanches Pompeo ^[2] ; Usberti, Felipe Rochelle ^[1] ; Anovazzi, Giovana ^[3] ; Pacheco, Leandro Edgar ^[2] ; Pansani, Taisa Nogueira ^[1] ; Leite, Maria Luisa ^[1] ; Hebling, Josimeri ^[3] ; Costa, Carlos Alberto de Souza ^[1] ; Soares, Diana Gabriela ^[2]

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Dept Operat Dent Endodont & Dent Mat, Bauru Sch Dent FOB, Al Dr Octavio Pinheiro Brizola 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 24, n. 2, p. 663-674, FEB 2020.
• Web of Science Citations: 2

Abstract

Objectives This study aimed to develop a porous chitosan-calcium-aluminate scaffold (CH-AlCa) in combination with a bioactive dosage of 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25VD), to be used as a bioactive substrate capable to increase the odontogenic potential of human dental pulp cells (HDPCs). Materials and methods The porous CH-AlCa was developed by the incorporation of an AlCa suspension into a CH solution under vigorous agitation, followed by phase separation at low temperature. Scaffold architecture, porosity, and calcium release were evaluated. Thereafter, the synergistic potential of CH-AlCa and 1 nM 1 alpha,25VD, selected by a dose-response assay, for HDPCs seeded onto the materials was assessed. Results The CH-AlCa featured an organized and interconnected pore network, with increased porosity in comparison with that of plain chitosan scaffolds (CH). Increased odontoblastic phenotype expression on the human dental pulp cell (HDPC)/CH and HDPC/CH-AlCa constructs in the presence of 1 nM 1 alpha,25VD was detected, since alkaline phosphatase activity, mineralized matrix deposition, dentin sialophosphoprotein/dentin matrix acidic phosphoprotein 1 mRNA expression, and cell migration were overstimulated. This drug featured a synergistic effect with CH-AlCa, since the highest values of cell migration and odontoblastic markers expression were observed in this experimental condition. Conclusions The experimental CH-AlCa scaffold increases the chemotaxis and regenerative potential of HDPCs, and the addition of low-dosage 1 alpha,25VD to this scaffold enhances the potential of these cells to express an odontoblastic phenotype. (AU)

• FAPESP's process: 17/20181-0 - Tissue Engineering and Biotechnology applyed to the development of calcium-containing macroporous scaffolds with surface nano-topography to modulate pulp-dentin complex regeneration
• Grantee: Ester Alves Ferreira Bordini Galvani
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/15674-5 - Association of tissue engineering techniques for mineralized tissue regeneration under degenerative inflammatory stimulus: analysis on 3D-culture perfusion bioreactor and animal inflammatory models
• Grantee: Diana Gabriela Soares dos Passos
• Support Opportunities: Research Grants - Young Investigators Grants