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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Coexpression and Transcriptome analyses identify active Apomixis-related genes in Paspalum notatum leaves

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Author(s):
de Oliveira, Fernanda A. [1] ; Vigna, Bianca B. Z. [2] ; da Silva, Carla C. [1] ; Favero, Alessandra P. [2] ; de Matta, Frederico P. [2] ; Azevedo, Ana L. S. [3] ; de Souza, Anete P. [4, 1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas UNICAMP, CBMEG, Campinas, SP - Brazil
[2] Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil
[3] Embrapa Gado Leite, Juiz De Fora, MG - Brazil
[4] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: BMC Genomics; v. 21, n. 1 JAN 28 2020.
Web of Science Citations: 0
Abstract

Background Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs. (AU)

FAPESP's process: 08/52197-4 - Genomic-assisted breeding of sugarcane: using molecular markers for understanding the genetic architecture of quantitative traits and to implement marker assisted selection
Grantee:Anete Pereira de Souza
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 18/18527-9 - Phylogenetic relationships and identification of markers for apomixis and species-specific Plicatula group in polyploid genomes of Paspalum
Grantee:Fernanda Ancelmo de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/24346-9 - Allelic variation analysis in genes of economic importance among hom(oe)ologous regions of sugarcane (Saccharum spp.) genome
Grantee:Carla Cristina da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral