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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen

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Author(s):
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Daniela P. C Tirapelli [1] ; Mucio Luiz de Assis Cirino [2] ; Camila A. Melo de Carvalho [3] ; Paulo Cezar Novais ; Rafael Zimak Figueredo [5] ; Lucas Barbosa Porsani [6] ; Isabella Stracieri Gula [7] ; Andressa Romualdo Rodrigues [8] ; Jairo Pinheiro da Silva [9] ; Adriana Lis Pereira [10] ; Fermino Sanches Lizarte Neto [11] ; Bruno Cesar Schimming [12] ; Maria de Fátima Galli Sorita Tazima [13] ; Valéria de Paula Sassoli Fazan [14] ; Carlos Gilberto Carlotti-Jr [15] ; Luis Fernando Tirapelli [16] ; Benedicto Oscar Colli [17]
Total Authors: 17
Affiliation:
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[1] USP. FMRP. Department of Surgery and Anatomy - Brasil
[2] USP. FMRP. Department of Surgery and Anatomy - Brasil
[3] USP. FMRP. Department of Surgery and Anatomy - Brasil
[5] USP. FMRP. Department of Surgery and Anatomy - Brasil
[6] USP. FMRP. Department of Surgery and Anatomy - Brasil
[7] USP. FMRP. Department of Surgery and Anatomy - Brasil
[8] USP. FMRP. Department of Surgery and Anatomy - Brasil
[9] USP. FMRP. Department of Surgery and Anatomy - Brasil
[10] USP. FMRP. Department of Surgery and Anatomy - Brasil
[11] USP. FMRP. Department of Surgery and Anatomy - Brasil
[12] UNESP. Department of Anatomy - Brasil
[13] USP. FMRP. Department of Surgery and Anatomy - Brasil
[14] USP. FMRP. Department of Surgery and Anatomy - Brasil
[15] USP. FMRP. Department of Surgery and Anatomy - Brasil
[16] USP. FMRP. Department of Surgery and Anatomy - Brasil
[17] USP. FMRP. Department of Surgery and Anatomy - Brasil
Total Affiliations: 17
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MORPHOLOGY; v. 38, n. 3, p. 523-529, 2020-06-00.
Abstract

SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved. (AU)

FAPESP's process: 05/00381-8 - Gene expression apoptosis after focal brain ischemia in rats and neutoprotective effect of hypotermia and ketoprofen
Grantee:Benedicto Oscar Colli
Support Opportunities: Regular Research Grants