Pinus residue/pectin-based composite hydrogels for the immobilization of beta-D-galactosidase

Author(s)

Cargnin, Mariana Aguiar ^[1] ; de Souza, Alana Gabrieli ^[2] ; de Lima, Giovanni Floriano ^[2] ; Gasparin, Bruna Carla ^[3] ; Rosa, Derval dos Santos ^[2] ; Paulino, Alexandre Tadeu ^{[3, 1]}

Affiliation

^[1] Santa Catarina State Univ, Postgrad Program Food Sci & Technol, Br 282, Km 574, BR-89870000 Pinhalzinho, SC - Brazil

^[2] Fed Univ ABC, Engn Modeling & Appl Social Sci Ctr CECS, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil

^[3] Santa Catarina State Univ, Dept Food & Chem Engn, Br 282, Km 574, BR-89870000 Pinhalzinho, SC - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 149, p. 773-782, APR 15 2020.
• Web of Science Citations: 0

Abstract

The aim of this work was to synthesize pinus residue/pectin-based composite hydrogels for the immobilization of beta-D-galactosidase. These hydrogels were synthesized via chemical crosslinking, and characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, thermal analysis, mechanical assays, X-ray diffraction, and swelling kinetics. The water absorption mechanism in the hydrogel networks occurs by non-Fickian transport. The beta-D-galactosidase immobilization capacities of the hydrogels containing 0, 5 and 10% of pinus residue were respectively 242.08 +/- 036, 18127 +/- 050 and 182.71 +/- 0.36 mg enzyme per g dried hydrogel. at pH 4.0 and after 600 min. These values were 182.99 +/- 0.41, 219.99 +/- 0.47 and 218.56 +/- 0.39 mg g(-1), respectively, at pH S.G. Pectin-based hydrogels demonstrated to be excellent solid supports for the immobilization of enzymes. beta-D-Galactosidase immobilized in pectin-based hydrogels could be applied in the hydrolysis of lactose contained in either dairy foods or lactose-intolerant individuals. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 18/11277-7 - PBAT films with cellulose nanostructures treated with essential oils encapsulated for active packaging
• Grantee: Derval dos Santos Rosa
• Support Opportunities: Regular Research Grants