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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of phages on soil bacterial communities and nitrogen availability under different assembly scenarios

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Author(s):
Braga, Lucas P. P. [1, 2] ; Spor, Ayme [2] ; Kot, Witold [3] ; Breuil, Marie-Christine [2] ; Hansen, Lars H. [3] ; Setubal, Joao C. [1] ; Philippot, Laurent [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[2] Univ Bourgogne Franche Comte, INRA, AgroSup Dijon, Agroecol, F-21000 Dijon - France
[3] Univ Copenhagen, Dept Plant & Environm Sci, Frederiksberg - Denmark
Total Affiliations: 3
Document type: Journal article
Source: MICROBIOME; v. 8, n. 1 APR 6 2020.
Web of Science Citations: 0
Abstract

Background Bacteriophages, the viruses infecting bacteria, are biological entities that can control their host populations. The ecological relevance of phages for microbial systems has been widely explored in aquatic environments, but the current understanding of the role of phages in terrestrial ecosystems remains limited. Here, our objective was to quantify the extent to which phages drive the assembly and functioning of soil bacterial communities. We performed a reciprocal transplant experiment using natural and sterilized soil incubated with different combinations of two soil microbial communities, challenged against native and non-native phage suspensions as well as against a cocktail of phage isolates. We tested three different community assembly scenarios by adding phages: (a) during soil colonization, (b) after colonization, and (c) in natural soil communities. One month after inoculation with phage suspensions, bacterial communities were assessed by 16S rRNA amplicon gene sequencing. Results By comparing the treatments inoculated with active versus autoclaved phages, our results show that changes in phage pressure have the potential to impact soil bacterial community composition and diversity. We also found a positive effect of active phages on the soil ammonium concentration in a few treatments, which indicates that increased phage pressure may also be important for soil functions. Conclusions Overall, the present work contributes to expand the current knowledge about soil phages and provide some empirical evidence supporting their relevance for soil bacterial community assembly and functioning. (AU)

FAPESP's process: 18/19247-0 - Unraveling the Role of Phages on the Evolution of Plant Beneficial and Pathogenic Rhizobacteria
Grantee:Lucas Palma Perez Braga
Support Opportunities: Scholarships in Brazil - Post-Doctoral