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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of apitoxin and melittin from Apis mellifera bee on Staphylococcus aureus strains

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Author(s):
Marques Pereira, Ana Flavia [1] ; Albano, Mariana [1] ; Bergamo Alves, Fernanda Cristina [1] ; Murbach Teles Andrade, Bruna Fernanda [1] ; Furlanetto, Alessandra [1] ; Mores Rall, Vera Lucia [1] ; dos Santos, Lucilene Delazari [2] ; Orsi, Ricardo de Oliveira [2] ; Fernandes Junior, Ary [1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ, Dept Microbiol & Immunol, BR-18618691 Botucatu, SP - Brazil
[2] Sao Paulo State Univ, CEVAP Ctr Study Venoms & Venomous Anim, BR-18610307 Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Microbial Pathogenesis; v. 141, APR 2020.
Web of Science Citations: 7
Abstract

The antibacterial activities of apitoxin, a venom produced by Apis mellifera bee, and melittin, an antimicrobial peptide from apitoxin, were tested against planktonic and biofilm states of Staphylococcus aureus methicillin-resistant (MRSA), including clinical, and enterotoxin-producing isolates. Also, the synergism of apitoxin and melittin in combination with oxacillin were evaluated as well. The induced morphological changes on S. aureus cells of both products were detected by transmission electronic microscopy (TEM). The minimum inhibitory concentration (MIC) values were 7.2 mu g/mL, and 6.7 mu g/mL, for apitoxin and melittin, respectively. The minimum bactericidal concentration (MBC) values were 28.7 mu g/mL, and 26 mu g/mL for apitoxin and melittin, respectively. The time-kill curve assays of apitoxin or melittin with oxacillin exhibited bactericidal synergism against MRSA isolates. TEM images showed cell distortion, cell disintegration with leakage of cytoplasmic content and loss of cytoplasm content. However, apitoxin and melittin did not interfere with staphylococcal enterotoxin production or release. Thus, apitoxin and melittin are potential agents against MRSA that can serve as possible models for new antibacterial drugs. (AU)

FAPESP's process: 15/14278-6 - The impact of natural compounds in bacteria of clinical interest
Grantee:Ary Fernandes Júnior
Support Opportunities: Regular Research Grants