Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Preparation for oxidative stress in Proceratophrys cristiceps (Anura, Odontophrynidae) naturally estivating in the Brazilian Caatinga

Full text
Author(s):
Moreira, Daniel C. [1, 2] ; Carvajalino-Fernandez, Juan M. [2, 3] ; Silva, Willianilson P. [4] ; Kuzniewsk, Felipe [2] ; Navas, Carlos A. [5] ; de Carvalho, Jose E. [6] ; Hermes-Lima, Marcelo [2]
Total Authors: 7
Affiliation:
[1] Univ Brasilia, Area Morfol, Fac Med, Brasilia, DF - Brazil
[2] Univ Brasilia, Dept Biot Celular, Brasilia, DF - Brazil
[3] Univ Coll Cundinamarca, Lab Adaptat Extreme Environm & Global Change Biol, Bogota - Colombia
[4] Univ Fed Rio Grande do Norte, Dept Bot & Zool, Natal, RN - Brazil
[5] Univ Sao Paulo, Dept Fisiol, Sao Paulo, SP - Brazil
[6] Univ Fed Sao Paulo, Dept Ecol & Biol Fvolut, Diadema, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Science of The Total Environment; v. 723, JUN 25 2020.
Web of Science Citations: 0
Abstract

Preparation for oxidative stress (POS), i.e., the buildup of endogenous antioxidants during metabolic depression or low oxygen stress conditions, has been observed in at least 8 animal phyla under controlled conditions in laboratory. Despite the expected implications on the endurance to extreme environments and ecosystem occupation, the extent to which POS occurs in animals under natural conditions remains unexplored. Therefore, we took advantage of the natural history of the Brazilian Caatinga's frog Proceratophrys cristiceps to investigate the modulation of endogenous antioxidants and redox balance in their skeletal muscle and to verify if POS occurs under natural conditions. Expectedly, estivating frogs had low levels of the oxidative metabolism enzymes. Citrate synthase and isocitrate dehydrogenase activities were 36% and 25% lower than those in active frogs respectively. We found an overall upregulation of antioxidants in estivating P. cristiceps. Reduced glutathione concentration was 61% higher in estivating frogs than that in active animals. During estivation the activities of the hydroperoxide detoxification enzymes catalase, glutathione peroxidase, and glutathione H2O2-peroxidase were 48%, 57%, and 78% greater than those during the rainy season. Moreover, estivating frogs had a 47% lower ratio of disulfide to total glutathione levels than active frogs. Our findings confirm the occurrence of `preparation for oxidative stress' in naturally estivating frogs and paves the way for further research on the redox biology of animals under natural settings. Such approach might reveal biochemical strategies under ecologically relevant scenarios. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/57712-4 - The National Institute of Comparative Physiological Research
Grantee:Augusto Shinya Abe
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
Grantee:Carlos Arturo Navas Iannini
Support Opportunities: Research Program on Global Climate Change - Thematic Grants