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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efficacy of a Protein Vaccine and a Conjugate Vaccine Against Co-Colonization with Vaccine-Type and Non-Vaccine Type Pneumococci in Mice

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Author(s):
Colichio, Gabriela B. C. [1] ; Oliveira, Giuliana S. [1] ; Rodrigues, Tasson C. [1] ; Oliveira, Maria Leonor S. [1] ; Miyaji, Eliane N. [1]
Total Authors: 5
Affiliation:
[1] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PATHOGENS; v. 9, n. 4 APR 2020.
Web of Science Citations: 0
Abstract

Widespread use of pneumococcal conjugate vaccines (PCVs) has led to substitution of vaccine-type (VT) strains by non-vaccine type (NVT) strains in nasopharyngeal carriage. We compared the efficacy of PCV13 and a nasal protein formulation containing pneumococcal surface protein A (PspA) adjuvanted with the whole-cell pertussis vaccine (wP) in the protection against co-colonization challenge models in mice with VT and NVT strains expressing different PspAs. Immunized mice were challenged with two different mixtures: i. VT4 (PspA3) + NVT33 (PspA1) and ii. VT23F (PspA2) + NVT15B/C (PspA4). Results from the first mixture showed a reduction in loads of VT4 strain in the nasopharynx of mice immunized with PCV13. A statistical difference between the loads of the VT and NVT strains was observed, indicating a competitive advantage for the NVT strain in PCV13-immunized animals. In the second mixture, no reduction was observed for the VT23F strain, probably due to low levels of anti-23F polysaccharide IgG induced by PCV13. Interestingly, a combination of the PspA formulation containing wP with PCV13 led to a reduction in colonization with both strains of the two mixtures tested, similar to the groups immunized nasally with wP or PspA plus wP. These results indicate that a combination of vaccines may be a useful strategy to overcome pneumococcal serotype replacement. (AU)

FAPESP's process: 16/09427-5 - Comparison of the immunization with a polysaccharide conjugate vaccine and protein antigens in the control of co-colonization with Streptococcus pneumoniae
Grantee:Eliane Namie Miyaji
Support Opportunities: Regular Research Grants