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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the articular cartilage in the knees of rats with induced arthritis treated with curcumin

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Author(s):
Nicoliche, Tiago [1] ; Maldonado, Diogo Correa [1] ; Faber, Jean [2] ; Pereira da Silva, Marcelo Cavenaghi [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Dept Morphol & Genet, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Neurol & Neurosurg, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 15, n. 3 MAR 12 2020.
Web of Science Citations: 0
Abstract

This study was designed to evaluate the anti-inflammatory effects of a curcumin treatment on the knee of rats with induced osteoarthritis. Fifteen adult rats were used and divided in three groups: the osteoarthritis group (OAG), control group (CG-without induction of osteoarthritis), and curcumin-treated osteoarthritis group (COAG). Osteoarthritis was induced in the right knee of rats in the OAG and COAG by administering an intra-articular injection of 1 mg of zymosan. Fourteen days after induction, 50 mg/kg curcumin was administered by gavage daily for 60 days to the COAG. After the treatment period, rats from all groups were euthanized. Medial femoral condyles were collected for light microscopy and immunohistochemical staining. The expression of SOX-5, IHH, MMP-8, MMP-13, and collagen 2 (Col2) was analyzed. The COAG exhibited an increase in the number of chondrocytes in the surface and middle layers compared with that of the OAG and CG, respectively. The COAG also showed a decrease in the thicknesses of the middle and deep layers compared with those of the OAG, and an increase in Col2 expression was observed in all articular layers (surface, middle, and deep) in the COAG compared with that in the OAG. SOX-5 expression was increased in the surface and deep layers of the COAG compared with those in the OAG and CG. Based on the results of this study, the curcumin treatment appeared to exert a protective effect on cartilage, as it did not result in an increase in cartilage thickness or in MMP8 and MMP-13 expression but led to increased IHH, Col2, and SOX-5 expression and the number of chondrocytes. (AU)

FAPESP's process: 18/11235-2 - Effects of diacerein, conjugate glucosamine/chondroitin and dental pulp stem cells on Wistar rats femur articular cartilage with osteoarthritis.
Grantee:Marcelo Cavenaghi Pereira da Silva
Support Opportunities: Regular Research Grants