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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unreliability of EDTA samples for measuring bioamine neurotransmitter levels in cats

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Author(s):
Ramos, Daniela [1] ; Reche-Junior, Archivaldo [1] ; Perez Brandao, Juliana Dineia [2] ; Arita, Danielle Yuri [2] ; Casarini, Dulce Elena [2] ; Mills, Daniel S. [3]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Med Clin, Sao Paulo - Brazil
[2] Sao Paulo Fed Univ, Fac Med, Nephrol Dept, Sao Paulo - Brazil
[3] Univ Lincoln, Sch Life Sci, Anim Behav Cognit & Welf Grp, Lincoln - England
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF FELINE MEDICINE AND SURGERY; v. 23, n. 2 JUN 2020.
Web of Science Citations: 0
Abstract

Objectives The aim of this study was to assess the reliability of an EDTA-based method for measuring cat blood bioamines. Methods Test 1 involved collecting blood samples from seven university laboratory cats. The samples were transferred to EDTA, heparin and plain tubes to determine concentrations of four bioamines (serotonin, dopamine, epinephrine {[}adrenaline] and norepinephrine {[}noradrenaline]). Correlation of measurements performed on EDTA plasma, with those performed on heparinized plasma or serum were assessed by intraclass correlation coefficients (ICCs). In test 2, blood samples from five owned cats were collected and stored in EDTA tubes and divided between duplicate Eppendorf tubes labeled as different cats for blinding purposes and analyzed independently for the same four bioamines as in test 1. Reliability of concentration determination for these duplicates was assessed by ICCs and coefficients of variation (CVs). Results In test 1, there was no significant correlation between the EDTA plasma serotonin and serum serotonin concentrations. There was also no significant correlation between EDTA plasma and heparin plasma concentrations for either epinephrine or norepinephrine. There was a statistically significant but weak correlation between EDTA plasma and heparin plasma dopamine concentrations. In test 2, there was no correlation for repeat-analyzed serotonin and epinephrine concentrations. Although there were statistically significant correlations for dopamine and norepinephrine, CVs for each analyte were in excess of 30%. Conclusions and relevance Before any further attempt is made to measure and report on neurotransmitter concentrations in domestic cats, it is essential that the robustness of the methodology is carefully validated and the data presented. (AU)