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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protective effects of desipramine on alveolar bone in experimental periodontitis

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Author(s):
Branco-de-Almeida, Luciana S. [1] ; Franco, Gilson C. N. [2] ; Castro, Myrella L. [3] ; Vieira, Mayana S. [4] ; Galvao-Moreira, V, Leonardo ; Cortelli, Sheila C. [5] ; Anbinder, Ana L. [6] ; Kawai, Toshihisa [7] ; Rosalen, Pedro L. [8]
Total Authors: 9
Affiliation:
[1] Univ Fed Maranhao, Post Grad Program Dent, Sao Luis, Maranhao - Brazil
[2] Univ Estadual Ponta Grossa, Dept Gen Biol, Ponta Grossa, Parana - Brazil
[3] Fac Sci Tocantins, Araguaina, Tocantins - Brazil
[4] Univ Ceuma, Post Grad Program Dent, Sao Luis, Maranhao - Brazil
[5] Univ Taubate, Nucleus Periodontal Res, Taubate, SP - Brazil
[6] Sao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil
[7] Nova Southeastern Univ, Coll Dent Med, Ft Lauderdale, FL 33314 - USA
[8] Univ Fed Alfenas, Biol Sci Grad Program, Rua Gabriel Monteiroda Silva 700, Predio E Sala, BR-37130001 Alfenas, MG - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Journal of Periodontology; v. 91, n. 12 JUN 2020.
Web of Science Citations: 0
Abstract

Background Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. Methods A total of 60 male Wistar rats were randomly assigned into three groups: 1) control: rats without ligature treated with vehicle (saline); 2) ligature: rats with ligature-induced periodontitis treated with vehicle; 3) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/d in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1 beta, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Results Alveolar bone loss was significantly reduced in the ligature + desipramine group (P < 0.05), whereas gingival collagen degradation was like the ligature group (P > 0.05). Desipramine administration downregulated mRNA expressions of IL-1 beta, iNOS, COX-2, and TIMP-1 when compared to vehicle alone in the ligature group (P < 0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P > 0.05); however, MMP-9 activity was lower in the group treated with desipramine (P < 0.05). Conclusion Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis. (AU)

FAPESP's process: 08/00566-6 - In vivo evaluation of the activity of fluoxetine and desipramine in host response in periodontal disease
Grantee:Luciana Salles Branco de Almeida
Support Opportunities: Scholarships in Brazil - Doctorate