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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High discrimination of Mycobacterium bovis isolates in Brazilian herds by spoligotyping

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Figueiredo Rocha, Vivianne Cambui [1] ; de Souza-Filho, Antonio Francisco [2] ; Ikuta, Cassia Yumi [2] ; Hildebrand e Grisi Filho, Jose Henrique [2] ; Issa, Marina de Azevedo [3] ; Pinto Coelho Mota, Pedro Moacyr [3] ; Ferre, Lucia de Juan [4, 5] ; Rodriguez, Lucas Dominguez [4, 5] ; Martinez, Beatriz Romero [4, 5] ; Heinemann, Marcos Bryan [2] ; Ferreira Neto, Jose Soares [2]
Total Authors: 11
[1] Inst Fed Educ Ciencia & Tecnol Paraiba, Sousa, Paraiba - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
[3] Minist Agr Pecuaria & Abastecimento, Lab Nacl Agr, Pedro Leopoldo, MG - Brazil
[4] Univ Complutense, Ctr Vigilancia Sanitaria Vet VISAVET, Madrid, Spain.Figueiredo Rocha, Vivianne Cambui, Inst Fed Educ Ciencia & Tecnol Paraiba, Sousa, Paraiba - Brazil
[5] Univ Complutense, Ctr Vigilancia Sanitaria Vet VISAVET, Madrid - Spain
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 0

Bovine tuberculosis is an infectious disease caused by Mycobacterium bovis (M. bovis), that leads to economic losses in infected herds and it is also considered an important zoonosis. The molecular typing methods of M. bovis isolates are fundamental for the bovine tuberculosis surveillance system, and spoligotyping is the standard genotyping technique for this species. Thus, the aim of the present study is to analyze the spatial and cluster distribution of M. bovis strains from several regions of Brazil through molecular typing. Spoligotyping technique was applied on 422 isolates identified as M. bovis, and Ripley's K function was used to perform the spatial and cluster analysis of each identified profile. Forty-three (43) different profiles were identified and spoligotype SB0121 was the most frequent and showed a uniform pattern in the spatial distribution while spoligotypes SB0295, SB1380 and SB1050 formed clusters. In addition, three novel spoligotype profiles (SB2361, SB2362, SB2364) were identified in different herds. In this perspective, it is believed that molecular identification and typing can significantly improve the performance of surveillance systems for bovine tuberculosis in Brazil. (AU)

FAPESP's process: 17/50190-1 - Studies to support the decision-making process within the PNCEBT and consequent implementation of public policies in Brazil
Grantee:José Soares Ferreira Neto
Support type: Research Grants - Research in Public Policies
FAPESP's process: 11/21514-7 - Molecular discrimination of Mycobacterium bovis and applications for tuberculosis surveillance
Grantee:José Soares Ferreira Neto
Support type: Regular Research Grants