Molecular discrimination of Mycobacterium bovis and applications for tuberculosis surveillance

• Grant number: 11/21514-7
• Support type: Regular Research Grants
• Duration: May 01, 2012 - April 30, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal researcher: José Soares Ferreira Neto
• Grantee: José Soares Ferreira Neto
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Bovine tuberculosis is an infectious disease caused by Mycobacterium bovis. The agent causes losses in infected herds and is an important zoonosis. Direct methods of diagnosis are important for a surveillance system for bovine tuberculosis. Aggregation of molecular methods brings greater efficiency for these systems, especially those methods with application in epidemiology. Among the molecular techniques are TB PCR Multiplex and Allele-Specific PCR (AS-PCR) for identification of BAAR isolated, and Variable Number Tandem Repeat, Spoligotyping and Multispacer Sequence Typing as techniques of fingerprint. Thus, this study aims to molecular identify of samples from several regions of Brazil by using molecular techniques TB Multiplex PCR and Allele-Specific PCR. The isolates identified as M. bovis will be submitted to VNTR, Spoligotyping and MST, such as fingerprint techniques. Discriminatory ability of these techniques will be compared and thematic maps will built with the genotypes of M. bovis in Brazil. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIGUEIREDO ROCHA, VIVIANNE CAMBUI; DE SOUZA-FILHO, ANTONIO FRANCISCO; IKUTA, CASSIA YUMI; HILDEBRAND E GRISI FILHO, JOSE HENRIQUE; ISSA, MARINA DE AZEVEDO; PINTO COELHO MOTA, PEDRO MOACYR; FERRE, LUCIA DE JUAN; RODRIGUEZ, LUCAS DOMINGUEZ; MARTINEZ, BEATRIZ ROMERO; HEINEMANN, MARCOS BRYAN; et al. High discrimination of Mycobacterium bovis isolates in Brazilian herds by spoligotyping. PREVENTIVE VETERINARY MEDICINE, v. 179, JUN 2020. (17/50190-1, 11/21514-7)