Evaluating the circadian rhythm and response to glucose addition in dispersed growth cultures of Neurospora crassa

Author(s)

Kelliher, Christina M. ^[1] ; Loros, Jennifer J. ^[2] ; Dunlap, Jay C. ^{[1, 3]}

Affiliation

^[1] Geisel Sch Med Dartmouth, Dept Mol & Syst Biol, Hanover, NH 03755 - USA

^[2] Geisel Sch Med Dartmouth, Dept Biochem & Cell Biol, Hanover, NH - USA

^[3] Loros, Jennifer J., Geisel Sch Med Dartmouth, Dept Biochem \& Cell Biol, Hanover, NH USA.Kelliher, Christina M., Geisel Sch Med Dartmouth, Dept Mol & Syst Biol, Hanover, NH 03755 - USA

• Document type: Journal article
• Source: FUNGAL BIOLOGY; v. 124, n. 5, p. 398-406, MAY 2020.
• Web of Science Citations: 1

Abstract

Work on the filamentous fungus Neurospora crassa has contributed to or pioneered many aspects of research on circadian clock mechanism, a process that is functionally conserved across eukaryotes. Biochemical assays of the fungal circadian clock typically involve growth in liquid medium where Neurospora forms a spherical ball of submerged mycelium. Here, we revive a method for dispersed growth of Neurospora in batch culture using polyacrylic acid as an additive to the medium. We demonstrate that dispersed growth cultures utilize more carbon than mycelial balls, but nonetheless retain a functional circadian clock. This culturing method is suited for use in circadian experiments where uniform exposure to nutrients and/or increased biomass is required. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
• Grantee: Drauzio Eduardo Naretto Rangel
• Support Opportunities: Organization Grants - Scientific Meeting