The Vta1 transcriptional regulator is required for microsclerotia melanization in Verticillium dahliae

Author(s)

Harting, Rebekka ^{[1, 2]} ; Hoefer, Annalena ^{[1, 2, 3]} ; Tran, Van-Tuan ; Weinhold, Lisa-Maria ^{[4, 5, 6]} ; Barghahn, Sina ^{[4, 5, 6]} ; Schlueter, Rabea ^[7] ; Braus, Gerhard H. ^{[4, 5, 6]}

Affiliation

^[1] Univ Gottingen, Inst Microbiol & Genet, Dept Mol Microbiol & Genet, Grisebachstr 8, D-37077 Gottingen - Germany

^[2] Gottingen Ctr Mol Biosci GZMB, Grisebachstr 8, D-37077 Gottingen - Germany

^[3] Van-Tuan Tran, Vietnam Natl Univ, VNU Univ Sci, Dept Microbiol, Hanoi, Vietnam.Harting, Rebekka, Univ Gottingen, Inst Microbiol & Genet, Dept Mol Microbiol & Genet, Grisebachstr 8, D-37077 Gottingen - Germany

^[4] Van-Tuan Tran, Univ Gottingen, Inst Microbiol & Genet, Dept Mol Microbiol & Genet, Grisebachstr 8, D-37077 Gottingen - Germany

^[5] Van-Tuan Tran, Gottingen Ctr Mol Biosci GZMB, Grisebachstr 8, D-37077 Gottingen - Germany

^[6] Van-Tuan Tran, Van-Tuan Tran, Vietnam Natl Univ, VNU Univ Sci, Dept Microbiol, Hanoi, Vietnam.Harting, Rebekka, Univ Gottingen, Inst Microbiol & Genet, Dept Mol Microbiol & Genet, Grisebachstr 8, D-37077 Gottingen - Germany

^[7] Ernst Moritz Arndt Univ Greifswald, Dept Biol, Imaging Ctr, Friedrich Ludwig Jahn Str 15, D-17489 Greifswald - Germany

• Document type: Journal article
• Source: FUNGAL BIOLOGY; v. 124, n. 5, p. 490-500, MAY 2020.
• Web of Science Citations: 0

Abstract

Many fungi are able to produce resting structures, which ensure survival and protect them against various stresses in their habitat such as exposure to UV light, temperature variations, drought as well as changing pH and nutrient conditions. Verticillium dahliae is a plant pathogenic fungus that forms melanized resting structures, called microsclerotia, for survival of time periods without a host. These highly stress resistant microsclerotia persist in the soil for many years and are therefore problematic for an effective treatment of the fungus. The Verticillium transcription activator of adhesion 1 (Vta1) was initially identified as one of several transcriptional regulators that rescue adhesion in non-adhesive Saccharomyces cerevisiae cells. Vta2 and Vta3 are required for early steps in plant infection and colonization and additionally control microsclerotia formation. Here, we show that Vta1 function is different, because it is dispensable for root colonization and infection. Vta1 is produced in the fungal cell during microsclerotia development. Analysis of the deletion mutant revealed that the absence of Vta1 allows microsclerotia production, but they are colorless and no more melanized. Vta1 is required for melanin production and activates transcription of melanin biosynthesis genes including the polyketide synthase encoding PKS1 and the lactase LAC1. The primary function of Vta1 in melanin production is important for survival of microsclerotia as resting structures of V. dahliae. (C) 2020 Published by Elsevier Ltd on behalf of British Mycological Society. (AU)

• FAPESP's process: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
• Grantee: Drauzio Eduardo Naretto Rangel
• Support Opportunities: Organization Grants - Scientific Meeting