Curcuma longaL. (turmeric),Rosmarinus officinalisL. (rosemary), andThymus vulgarisL. (thyme) extracts aid murine macrophages (RAW 264.7) to fightStreptococcus mutansduring in vitro infection

Author(s)

Camargo, Samira Esteves Afonso ^[1] ; de Oliveira, Luciane Dias ^[2]

Affiliation

^[1] Univ Florida, Coll Dent, Dept Restorat Dent Sci, Gainesville, FL 32610 - USA

^[2] Sao Paulo State Univ UNESP, Dept Biosci & Oral Diag, Inst Sci & Technol, Ave Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 202, n. 8 JUN 2020.
• Web of Science Citations: 0

Abstract

Finding an effective alternative way to aid defense cells to fightStreptococcus mutanswas the main goal of this study. The effect of plant extracts fromCurcuma longaL. (turmeric),Rosmarinus officinalisL. (rosemary), andThymus vulgarisL. (thyme) was evaluated on murine macrophages (RAW 264.7) infected byS. mutans.Minimum inhibitory concentration (MIC) of the extracts was determined. Macrophages were infected byS. mutansand treated with each extract. From the supernatants, it was measured nitric oxide (NO) level. Posteriorly, RAW 264.7 were lysed to expose living and phagocytosed bacteria. Cytotoxicity was checked by lysosomal activity analysis, using neutral red assay. Each extract helped RAW 264.7 to eliminateS. mutansduring infection, as observed by a significant bacterial reduction. Significant cell viability was also found. Besides, an increased production of NO was verified usingR. officinalisL. andT. vulgarisL. extracts. The evaluated extracts demonstrated an effective action to assist RAW 264.7 to fightS. mutansduring infection. (AU)

• FAPESP's process: 15/08776-3 - ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY, CYTOTOXICITY AND GENOTOXICITY OF DIFFERENT NATURAL EXTRACTS
• Grantee: Luciane Dias de Oliveira
• Support Opportunities: Regular Research Grants