DNA damage is inversely associated to blood levels of DHA and EPA fatty acids in Brazilian children and adolescents

Author(s):Show less -

Barros, Tamiris Trevisan de ^{[1, 2]} ; Venancio, Vinicius de Paula ^[3] ; Hernandes, Livia Cristina ^[3] ; Greggi Antunes, Lusania Maria ^[3] ; Hillesheim, Elaine ^{[1, 2]} ; Salomao, Roberta Garcia ^{[1, 2]} ; Mathias, Mariana Giaretta ^{[1, 2]} ; Coelho-Landell, Carolina Almeida ^{[1, 2]} ; Donega Toffano, Roseli Borges ^{[1, 2]} ; Ribeiro do Vale Almada, Maria Olimpia ^{[1, 2]} ; Camelo-Junior, Jose Simon ^{[1, 2]} ; Moco, Sofia ^[4] ; Ued, Fabio da Veiga ^{[1, 2]} ; Kaput, Jim ^[4] ; Monteiro, Jacqueline Pontes ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Pediat, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Hlth Sci, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Nestle Inst Hlth Sci, Lausanne - Switzerland

• Document type: Journal article
• Source: FOOD & FUNCTION; v. 11, n. 6, p. 5115-5121, JUN 1 2020.
• Web of Science Citations: 0

Abstract

This study aimed to investigate the association between DNA damage and blood levels of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), retinol, beta-carotene and riboflavin in Brazilian children and adolescents. Subjects (n= 140) were healthy boys and girls aged 9 to 13 years in Ribeirao Preto (SP, Brazil). Data collection included anthropometry, assessment of energy intake and blood sampling. DNA damage was evaluated by single-cell gel electrophoresis (comet assay). Principal component analysis (PCA) was used to verify associations between blood concentrations of vitamins, polyunsaturated fatty acids and DNA damage. Multiple regression analyses,k-means cluster, and analysis of covariance (ANCOVA), adjusted for confounding variables such as age, sex, energy intake, body mass index and total cholesterol (when needed), were applied to confirm the associations. PCA explained 69.4% of the inverse relationships between DNA damage and blood levels of DHA, EPA, retinol, and beta-carotene. Results were confirmed by ANCOVA and multiple regression analyses for DHA and EPA. In conclusion, omega-3-fatty acids were inversely associated with DNA damage in Brazilian children and adolescents and may be a protective factor against the development of future diseases. (AU)

• FAPESP's process: 12/20421-8 - A new strategy to analyze gene-nutrient interaction in children and adolescents
• Grantee: Jacqueline Pontes Monteiro
• Support Opportunities: Regular Research Grants