Modular assembly of yeast mitochondrial ATP synthase and cytochrome oxidase

Author(s)

Ribeiro Franco, Leticia Veloso ^{[1, 2]} ; Su, Chen Hsien ^[2] ; Tzagoloff, Alexander ^[2]

Affiliation

^[1] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508000 Sao Paulo - Brazil

^[2] Columbia Univ, Dept Biol Sci, New York, NY 10027 - USA

• Document type: Review article
• Source: Biological Chemistry; v. 401, n. 6-7, p. 835-853, MAY 2020.
• Web of Science Citations: 0

Abstract

The respiratory pathway of mitochondria is composed of four electron transfer complexes and the ATP synthase. In this article, we review evidence from studies of Saccharomyces cerevisiae that both ATP synthase and cytochrome oxidase (COX) are assembled from independent modules that correspond to structurally and functionally identifiable components of each complex. Biogenesis of the respiratory chain requires a coordinate and balanced expression of gene products that become partner subunits of the same complex, but are encoded in the two physically separated genomes. Current evidence indicates that synthesis of two key mitochondrial encoded subunits of ATP synthase is regulated by the F-1 module. Expression of COX1 that codes for a subunit of the COX catalytic core is also regulated by a mechanism that restricts synthesis of this subunit to the availability of a nuclear-encoded translational activator. The respiratory chain must maintain a fixed stoichiometry of the component enzyme complexes during cell growth. We propose that high-molecular-weight complexes composed of Cox6, a subunit of COX, and of the Atp9 subunit of ATP synthase play a key role in establishing the ratio of the two complexes during their assembly. (AU)

• FAPESP's process: 19/16015-3 - ATCO1, a mitochondrial master regulator of ATP Synthase and Cytochrome Oxidase biogenesis?
• Grantee: Leticia Veloso Ribeiro Franco
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor