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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diuretic and Renal Protective Effect of Kaempferol 3-O-Alpha-L-rhamnoside (Afzelin) in Normotensive and Hypertensive Rats

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Author(s):
Cechinel-Zanchett, Camile Cecconi [1] ; Bolda Mariano, Luisa Nathaprimelia [1] ; Boeing, Thaise [1] ; da Costa, Juliana de Carvalho [2] ; da Silva, Luisa Mota [1] ; Bastos, Jairo K. [2] ; Cechinel-Filho, Valdir [1] ; de Souza, Priscila [1]
Total Authors: 8
Affiliation:
[1] Univ Vale Itajai, UNIVALI, Nucleo Invest Quim Farmaceut NIQFAR, Programa Posgrad Ciencias Farmaceut, Itajai, SC - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Natural Products; v. 83, n. 6, p. 1980-1989, JUN 26 2020.
Web of Science Citations: 8
Abstract

Our previous study showed that kaempferitrin, the main flavonoid from Bauhinia forficata Link leaves, induces diuresis and saluresis when orally given to rats. Since afzelin (AFZ) and kaempferol (KFL) are active compounds from the biometabolism of kaempferitrin, the diuretic and renal protective properties of these two compounds were evaluated. While the acute treatment with AFZ evoked a diuretic action associated with an increase in Cl- excretion and a Ca2+-sparing effect, KFL did not present any activity. The pretreatment with a muscarinic receptor blocker or with an inhibitor of the cyclooxygenase fully avoided AFZ-induced diuresis. AFZ also induced a prolonged (7-day treatment) diuretic effect in normotensive (NTR) and hypertensive rats (SHR), with an increase of urinary Na+ and Cl- excretion, while it decreased the elimination of Ca2+. AFZ was able to decrease ROS and nitrite generation on kidney homogenates in comparison with the SHR group treated with the vehicle, as well as mitigated the changes in the renal corpuscle region (glomerulus and Bowman's capsule). Moreover, AFZ significantly reduced calcium oxalate crystal formation in urine, with inhibition rates of 41% for the NTR and 92% for the SHR group. Taken together, this study shows that AFZ exerts acute and prolonged diuretic effects plus protective renal properties. (AU)

FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support type: Research Projects - Thematic Grants