| Full text | |
| Author(s): |
Imperador, Carlos Henrique L.
[1]
;
Bardella, Vanessa B.
[2]
;
dos Anjos, Eli Heber M.
[1]
;
Rodrigues, Vera L. C. C.
[3]
;
Cabral-de-Mello, Diogo C.
[2]
;
Mello, Maria Luiza S.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Campinas Unicamp, Inst Biol, Dept Struct & Funct Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] State Univ Sao Paulo Unesp, Inst Biosci, Dept Biol, Ave 24-A, 1515, BR-13506900 Rio Claro, SP - Brazil
[3] Superintendence Control Endem Dis SUCEN, Rua Afonso Pessini 86, BR-13845206 Mogi Guacu, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Microscopy and Microanalysis; v. 26, n. 3, p. 567-574, JUN 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Constitutive heterochromatin typically exhibits low gene density and is commonly found adjacent or close to the nuclear periphery, in contrast to transcriptionally active genes concentrated in the innermost nuclear region. InTriatoma infestanscells, conspicuous constitutive heterochromatin forms deeply stained structures named chromocenters. However, to the best of our knowledge, no information exists regarding whether these chromocenters acquire a precise topology in the cell nuclei or whether their 18S rDNA, which is important for ribosome function, faces the nuclear center preferentially. In this work, the spatial distribution of fluorescent Feulgen-stained chromocenters and the distribution of their 18S rDNA was analyzed in Malpighian tubule cells ofT. infestansusing confocal microscopy. The chromocenters were shown to be spatially positioned relatively close to the nuclear periphery, though not adjacent to it. The variable distance between the chromocenters and the nuclear periphery suggests mobility of these bodies within the cell nuclei. The distribution of 18S rDNA at the edge of the chromocenters was not found to face the nuclear interior exclusively. Because the genome regions containing 18S rDNA in the chromocenters also face the nuclear periphery, the proximity of the chromocenters to this nuclear region is not assumed to be associated with overall gene silencing. (AU) | |
| FAPESP's process: | 15/10356-2 - Action of valproic acid on chromatin structure and function |
| Grantee: | Maria Luiza Silveira Mello |
| Support Opportunities: | Research Projects - Thematic Grants |