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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mast cells exhibit intracellular microbicidal activity againstAggregatibacter actinomycetemcomitans

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Author(s):
de Lima, Heliton G. [1] ; Pinke, Karen H. [1] ; Lopes, Marcelo M. R. [2] ; Buzalaf, Camila P. [3] ; Campanelli, Ana Paula [3] ; Lara, Vanessa S. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Integrated Res Ctr, Bauru, SP - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PERIODONTAL RESEARCH; v. 55, n. 5 JUL 2020.
Web of Science Citations: 0
Abstract

Background and Objective Several studies have demonstrated that mast cells are equipped with versatile tools to combat and kill bacteria. Additionally, mast cells produce and secrete a variety of mediators, which either regulate the host's immune system or directly attack bacteria. In this study, the intracellular microbicidal capacity of mast cells againstAggregatibacter actinomycetemcomitanswas evaluated. Methods Murine mast cells were challenged in vitro withA actinomycetemcomitansfor 3, 5, 10, and 24 hours. Subsequently, the colony-forming units were counted. Additionally, the production and release of nitric oxide and hydrogen peroxide were analyzed by DAF-FM diacetate, the Griess reaction, and the Amplex Red kit, respectively. Cell death was evaluated using FITC Annexin V and propidium iodide staining. Results Mast cells are able to efficiently eliminate periodontopathogen, with best results after 10 hours of intracellular challenge. The production/release of nitric oxide-and to a lesser extent of hydrogen peroxide-by mast cells was in agreement with its microbicidal capacity. Ninety percent of the mast cells maintained their cellular viability even after 24 hours of bacterial challenge. Conclusions This is-to the best of our knowledge-the first report to describe the intracellular microbicidal activity of mast cells againstA actinomycetemcomitans, concerning the production and release of potentially bactericidal substances. Further, the low number of cell deaths confirms that the decreased number of colony-forming units was due to the higher antimicrobial activity of mast cells. The results highlight the importance of these cells in the defense mechanisms of biofilm-induced periodontal disease. (AU)

FAPESP's process: 09/14152-1 - Defense mechanisms of mast cells against Candida albicans: involvement of Toll-like 2 receptor in the production of cytokines TNF-alpha and IL-10, and the phagocytic and antimicrobial activities
Grantee:Vanessa Soares Lara
Support Opportunities: Regular Research Grants