| Full text | |
| Author(s): Show less - |
Amorim, Mariene Ribeiro
[1]
;
Pontelli, Marjorie Cornejo
[2]
;
de Souza, Gabriela Fabiano
[1]
;
Muraro, Stefanie Primon
[1]
;
Toledo-Teixeira, Daniel A.
[1]
;
Forato, Julia
[1]
;
Bispo-dos-Santos, Karina
[1]
;
Barbosa, Natalia S.
[2]
;
Martini, Matheus Cavalheiro
[1]
;
Parise, Pierina Lorencini
[1]
;
Vieira, Aline
[1]
;
Milanez, Guilherme Paier
[1]
;
Pinto daSilva, Luis Lamberti
[2]
;
Lalwani, Pritesh Jaychand
[3]
;
Farias, Alessandro Santos
[1]
;
Ramirez Vinolo, Marco Aurelio
[1]
;
Sesti-Costa, Renata
[4]
;
Arruda, Eurico
[2]
;
Proenca-Modena, Jose Luiz
[1]
Total Authors: 19
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083862 Campinas - Brazil
[2] Univ Sao Paulo, Dept Cell Biol & Virol Res Ctr, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto - Brazil
[3] Fiocruz Amazonia, Leonidas & Maria Deane Inst ILMD, BR-69057070 Manaus, Amazonas - Brazil
[4] Univ Estadual Campinas, Med Sch, Hematol & Hemotherapy Ctr, BR-13083887 Campinas - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Viruses-Basel; v. 12, n. 7 JUL 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Oropouche orthobunyavirus(OROV) is an emerging arbovirus with a high potential of dissemination in America. Little is known about the role of peripheral blood mononuclear cells (PBMC) response during OROV infection in humans. Thus, to evaluate human leukocytes susceptibility, permissiveness and immune response during OROV infection, we applied RNA hybridization, qRT-PCR and cell-based assays to quantify viral antigens, genome, antigenome and gene expression in different cells. First, we observed OROV replication in human leukocytes lineages as THP-1 monocytes, Jeko-1 B cells and Jurkat T cells. Interestingly, cell viability and viral particle detection are maintained in these cells, even after successive passages. PBMCs from healthy donors were susceptible but the infection was not productive, since neither antigenome nor infectious particle was found in the supernatant of infected PBMCs. In fact, only viral antigens and small quantities of OROV genome were detected at 24 hpi in lymphocytes, monocytes and CD11c(+)cells. Finally, activation of the Interferon (IFN) response was essential to restrict OROV replication in human PBMCs. Increased expression of type I/III IFNs, ISGs and inflammatory cytokines was detected in the first 24 hpi and viral replication was re-established after blocking IFNAR or treating cells with glucocorticoid. Thus, in short, our results show OROV is able to infect and remain in low titers in human T cells, monocytes, DCs and B cells as a consequence of an effective IFN response after infection, indicating the possibility of leukocytes serving as a trojan horse in specific microenvironments during immunosuppression. (AU) | |
| FAPESP's process: | 16/00194-8 - Pathogenesis and neurovirulence of emerging viruses in Brazil |
| Grantee: | José Luiz Proença Módena |
| Support Opportunities: | Research Grants - Young Investigators Grants |