Identification of cross-reactive human single-chain variable fragments against phospholipases A(2) from Lachesis muta and Bothrops spp venoms

Author(s)

Campos, Lucas B. ^[1] ; Pucca, Manuela B. ^{[2, 3]} ; Silva, Luciano C. ^{[1, 4]} ; Pessenda, Gabriela ^[1] ; Filardi, Bruno A. ^[1] ; Cerni, Felipe A. ^{[2, 5]} ; Oliveira, Isadora S. ^[5] ; Laustsen, Andreas H. ^[2] ; Arantes, Eliane C. ^[5] ; Barbosa, Jose E. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil

^[2] Tech Univ Denmark, Dept Biotechnol & Biomed, Lyngby - Denmark

^[3] Univ Fed Roraima, Med Sch, Ave Capitao Ene Garcez 2413, Boa Vista, Parana - Brazil

^[4] Univ Toronto, Toronto Recombinant Antibody Ctr, Donnelly Ctr, Toronto, ON - Canada

^[5] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Biomol Sci, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Toxicon; v. 184, p. 116-121, SEP 2020.
• Web of Science Citations: 0

Abstract

Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A(2) from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay. (AU)

• FAPESP's process: 17/04724-4 - Improving of human fragment antibodies (scFVs) specific for animals' venoms
• Grantee: Eliane Candiani Arantes Braga
• Support type: Regular Research Grants
• FAPESP's process: 17/14035-1 - Improving of human fragment antibodies (scFvs) specific for animals' venoms
• Grantee: Felipe Augusto Cerni
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 18/14158-9 - Improving of human fragment antibodies (scFvs) specific for animals' venoms
• Grantee: Felipe Augusto Cerni
• Support type: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 17/03580-9 - Biochemical, structural and functional evaluation of a phosphodiesterase from Crotalus durissus collilineatus venom
• Grantee: Isadora Sousa de Oliveira
• Support type: Scholarships in Brazil - Doctorate