Determination, characterization and modeling of aqueous biphasic systems composed of propylammonium-based ionic liquids and phosphate salts

Author(s)

Mussagy, Cassamo U. ^{[1, 2]} ; Tabanez, Naiara L. ^[2] ; Farias, Fabiane O. ^[3] ; Kurnia, Kiki A. ^[4] ; Mafra, Marcos R. ^[3] ; Pereira, Jorge F. B. ^{[2, 5]}

Affiliation

^[1] Univ Aveiro, Dept Chem, CICECO Aveiro Inst Mat, P-3810193 Aveiro - Portugal

^[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Engn Bioproc & Biotechnol, BR-14800903 Araraquara, SP - Brazil

^[3] Fed Univ Parana UFPR, Polytech Ctr, Dept Chem Engn, Curitiba, Parana - Brazil

^[4] Univ Airlangga, Fac Fisheries & Marine, Dept Marine, Jalan Mulyorejo Kampus C, Surabaya 60115 - Indonesia

^[5] Univ Coimbra, CIEPQPF, Dept Chem Engn, Rua Silvio Lima, Polo 2 Pinhal de Marrocos, P-3030790 Coimbra - Portugal

• Document type: Journal article
• Source: Chemical Physics Letters; v. 754, SEP 2020.
• Web of Science Citations: 0

Abstract

Ionic liquids (ILs)-based aqueous biphasic systems (ABS) are efficient platforms for the extraction and purification of biomolecules. To find more biocompatible ABS, propylammonium-based ILs, phosphate salts and water were mixed at three temperatures, evaluating the influence of IL and temperature on the liquid-liquid equilibria (LLE). The biphasic region increases with the increase of IL' anionic/cationic chain and decreases with the increase of temperature. There is good agreement between the IL' ability to form ABS and the hydrogen bonding interaction predicted by COSMO-RS. The subtle balance of hydrogen bonding between salt anion-water and IL anion-water governs the phase separation. (AU)

• FAPESP's process: 15/50058-0 - Characterization and modelling of aqueous two-phase systems (ATPS) composed of ionic liquids (ILs) and polymers: a boost towards develiping sustainable biopharmaceutical separion
• Grantee: Jorge Pereira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase)
• Grantee: Jorge Pereira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/19793-3 - Optimization and scale-up of novel Ionic-Liquid-based purification processes for recombinant green fluorescent protein produced by Escherichia coli "GFPurIL"
• Grantee: Sandro Roberto Valentini
• Support Opportunities: Regular Research Grants