Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase

Author(s)

Sepulchro, Ana Gabriela Veiga ^[1] ; Pellegrini, Vanessa O. A. ^[1] ; Briganti, Lorenzo ^[1] ; de Araujo, Evandro A. ^[1] ; de Araujo, Simara S. ^[1] ; Polikarpov, Igor ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 247, NOV 1 2020.
• Web of Science Citations: 5

Abstract

Enzymatic transformation of xylans into renewable fuels and value-added products is mediated by xylanases. Here we describe the biochemical and X-ray structural characterization of Thermobacillus composti GH10 xylanase (TcXyn10A) at 2.1 angstrom resolution aiming to unravel details of its recognition of glucurono- and arabinoxylan at a molecular level. TcXyn10A improves the efficiency of pretreated lignocellulosic biomass hydrolysis by a commercial enzyme cocktail causing a 15.35 % increase in xylose release and 4.38 % glucose release after 24 h of reaction. The enzyme releases predominantly xylobiose and xylotriose, as well as MeGlcA3 x 3 (from beechwood glucuronoxylan) and a range of decorated xylooligosaccharides (XOS) from rye arabinoxylan, with Ara2 x 2 being the major product. The enzyme liberates XOS with the yields of 29.09 % for beechwood glucuronoxylan and 16.98 % for rye arabinoxylan. Finally, TcXyn10A has a high thermal stability, halotolerance, and resistance to ethanol, biochemical properties that can be desirable for a number of industrial applications. (AU)

• FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
• Grantee: Igor Polikarpov
• Support Opportunities: Research Projects - Thematic Grants