Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties

Author(s)

Rocha Neto, J. B. M. ^[1] ; Gomes Neto, R. J. ^[1] ; Bataglioli, R. A. ^[1] ; Taketa, T. B. ^[1] ; Pimentel, S. B. ^[2] ; Baratti, M. O. ^[2] ; Costa, C. A. R. ^[3] ; Carvalho, H. F. ^[2] ; Beppu, M. M. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, BR-13083852 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Cell Biol, BR-13083970 Campinas, SP - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 158, p. 197-207, SEP 1 2020.
• Web of Science Citations: 1

Abstract

This paper explores different film assembly conditions of the polyelectrolyte solutions of hyaluronan (HA) and chitosan (CHI), as well as both substrate and cell surface modifications, to investigate PC3 cells adhesion properties. UV-Visible, AFM-IR and Zeta potential techniques indicate that the solution ionic strength is a relevant parameter to modulate the free carboxylic groups of HA on the film surface. In addition, capacitive coupling measurements suggest that assembly conditions that favor surface charge mobility inhibit cell adhesion due to polymer rearrangements that support non-specific electrostatic interactions of positively charged CHI residues and the negatively charged cell moieties, rather than specific CD44-hyaluronan interactions. Moreover, the PC3 cells treatment with hyaluronidase and anti-CD44 antibody also highlighted the importance of CD44 binding site availability on the tumor cell adhesion properties. Finally, the conjugation of wheat germ agglutinin on the film surface proved to be a suitable strategy to boost the PC3 cell adhesion properties. Our results reveal the remarkable capacity of HA/CHI films to modulate cell-substrate properties, which pave the road for the development of surfaces suitable for several applications based on biosensing. (c) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/19976-6 - Development of functional thin films via layer-by-layer technique aiming selective cell capture for therapeutic and diagnostic purposes
• Grantee: Marisa Masumi Beppu
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/10193-9 - New mechanisms for controlling triggered release of anticancer drugs in cellular backpacks
• Grantee: Rogério Aparecido Bataglioli
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 13/05135-1 - Nanostructured antimicrobial coatings containing chitosan produced by self-assembly technique (layer-by-layer) for textile substrates
• Grantee: Thiago Bezerra Taketa
• Support Opportunities: Scholarships in Brazil - Doctorate