| Full text | |
| Author(s): |
Ferrara, Taise F. S.
[1]
;
Schneider, Vanessa K.
[2]
;
Lima, Patricia Silva
[1]
;
Bronze, Fellipe S.
[1]
;
Machado, Marcelo F. M.
[1]
;
Henrique-Silva, Flavio
[2]
;
Soares-Costa, Andrea
[2]
;
Carmona, Adriana K.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biofis, BR-04039032 Sao Paulo - Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | International Journal of Biological Macromolecules; v. 158, p. 375-383, SEP 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Huanglongbing (HLB) isa devastating citrus disease associated with Candidatus Liberibacter asiaticus (CLas) and is transmitted by the psyllid Diaphorina citri Kuwayama. Diaphorina citri belongs to Hemiptera order, which has cysteine peptidases as the most abundant proteolytic enzymes present in digestive tract. As cysteine peptidases are involved in different insect development processes, this class of enzymes has acquired biotechnological importance. In this context, we identified a cathepsin L-like (DCcathL1) from the Diaphorina citri transcriptome database and expressed the enzyme in E. coli. Quantitative real-time RT-PCR was conducted to determine DCcathL1 gene expression in different parts and developmental phases of the insect. We observed that DCcathL1 expression in the gut was 2.59 and 2.87-fold higher than in the head and carcass, respectively. Furthermore, DCcathL1 expression was greater in eggs than in nymphs and adults, suggesting a putative role of the enzyme in the embryonic development. In addition, enzymatic inhibitory activity using four recombinant Citrus cystatins were per formed. Among them, CsinCPI-2 was the strongest DCcathL1 inhibitor with a K-i value of 0.005 nM. Our results may contribute in the development of strategies for D. citri control, such as silencing the DCcathL1 gene and the use of transgenic plants that overexpress peptidase inhibitors. (c) 2020 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 16/02412-2 - Identification and characterization of targets for insect control Diaphorina citri, vector citrus disease Huanglongbing |
| Grantee: | Taíse Fernanda da Silva Ferrara |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/11861-2 - Involvement of Plasmodium proteases in the pathophysiology of malaria |
| Grantee: | Adriana Karaoglanovic Carmona |
| Support Opportunities: | Regular Research Grants |