Spawning induction and embryonic development ofSalminus hilarii(Characiformes: Characidae)

Author(s)

Araujo, Bruno Cavalheiro ^[1] ; Mello, Paulo Henrique ^[2] ; Moreira, Renata Guimaraes ^[3] ; Silva Hilsdorf, Alexandre Wagner ^[1] ; Marques, Victor Hugo ^[3] ; Honji, Renato Massaaki ^[4]

Affiliation

^[1] Univ Mogi das Cruzes, Nucleo Integrad Biotecnoi, Av Dr Candido Xavier de Almeida Souza 200, BR-08780911 Mogi Das Cruzes, SP - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho CA, Ctr Aquicultura, Via Acesso Prof Paulo Donato Castaane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao, Trav 14, 321, BR-05508090 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Ctr Biol Marinha, Rodovia Manoel Hipolito do Rego, Km 131, 5, BR-11612109 Sao Sebastiao, SP - Brazil

• Document type: Journal article
• Source: ZYGOTE; v. 28, n. 5, p. 377-387, OCT 2020.
• Web of Science Citations: 0

Abstract

The present study aimed to evaluate two different methods of artificial reproduction induction, and characterize the embryonic development ofSalminus hilarii. Different than observed for other tropical fish species, artificial reproduction induction followed by hand-stripping of gametes was considered unfeasible forS.hilarii, as no gamete fertilization was observed. However, females that were induced and spawned naturally presented a fertilization rate of 65.64 +/- 0.54%. With a mean temperature of 26.20 +/- 0.90oC it was possible to clearly distinguish a large perivitelline space at 14 min after fertilization (AF) and at 49 min AF more than 50% of the embryos presented two blastomeres, and these cleavages occurred until 1 h 54 min AF. The gastrula phase was characterized at 3 h 56 min AF, and blastopore closure was observed at 8 h 31 min AF. At 9 h 1 min, organogenesis started, with a clear distinction of the yolk mass, embryonic axis, cephalic and caudal regions; at 11 h 51 min AF the embryos already had advanced segmentation and a free tail. Total hatching occurred at 21 h 17 min and after opening the mouth, which occurred at 33 h 9 min, the larvae ofS.hilariipresented a strong and characteristic cannibalism. This information can be considered fundamental to improvingS.hilariiproduction in captivity and for collaboration with a conservation programme in the upper Tiete river basin. (AU)

• FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
• Grantee: Carlos Arturo Navas Iannini
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants
• FAPESP's process: 17/06765-0 - Reproductive physiology of the Dusky Grouper Epinephelus marginatus (Perciformes: Serranidae) in captivity: hormonal therapies applied to the induction of sexual maturation in pre-spawning phase
• Grantee: Renato Massaaki Honji
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 08/57687-0 - Effects of global climate change of the Brazilian fauna: a conservation physiology approach
• Grantee: Carlos Arturo Navas Iannini
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants