Interaction Between NOS3 and HMOX1 on Antihypertensive Drug Responsiveness in Preeclampsia

Author(s)

Valeria Cristina Sandrim ^[1] ; Marcelo Rizzatti Luizon ^[2] ; Eliane Pilan ^[3] ; Mayara Caldeira-Dias ^[4] ; Fernanda Borchers Coeli-Lacchini ^[5] ; Georgia Kors ^[6] ; Iuly Berndt ^[7] ; Riccardo Lacchini ^[8] ; Ricardo Carvalho Cavalli ^[9]

Affiliation

^[1] Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Department of Pharmacology - Brasil

^[2] Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Department of Genetics, Ecology and Evolution - Brasil

^[3] Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Department of Pharmacology - Brasil

^[4] Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Department of Pharmacology - Brasil

^[5] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Internal Medicine - Brasil

^[6] Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Department of Pharmacology - Brasil

^[7] Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Department of Pharmacology - Brasil

^[8] Universidade de São Paulo. Escola de Enfermagemde Ribeirão Preto. Department of Psychiatric Nursing and Human Sciences - Brasil

^[9] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Department of Gynecology and Obstetrics - Brasil

• Document type: Journal article
• Source: Revista Brasileira de Ginecologia e Obstetrícia; v. 42, n. 8, p. 460-467, 2020-09-25.

Abstract

Abstract Objective We examined the interaction of polymorphisms in the genes heme oxygenase- 1 (HMOX1) and nitric oxide synthase (NOS3) in patients with preeclampsia (PE) as well as the responsiveness to methyldopa and to total antihypertensive therapy. Methods The genes HMOX1 (rs2071746, A/T) and NOS3 (rs1799983, G/T) were genotyped using TaqMan allele discrimination assays (Applied Biosystems, Foster City, CA, USA ), and the levels of enzyme heme oxygenase-1 (HO-1) were measured using enzyme-linked immunosorbent assay (ELISA). Results We found interactions between genotypes of the HMOX-1 and NOS3 genes and responsiveness tomethyldopa and that PE genotyped as AT presents lower levels of protein HO-1 compared with AA. Conclusion We found interactions between the HMOX-1 and NOS3 genes and responsiveness to methyldopa and that the HMOX1 polymorphism affects the levels of enzyme HO-1 in responsiveness to methyldopa and to total antihypertensive therapy. These data suggest impact of the combination of these two polymorphisms on antihypertensive responsiveness in PE. (AU)

• FAPESP's process: 15/20461-8 - Role of NRF2 and HO-1 in preeclampsia: polymorphisms, circulating factors and in vitro assays
• Grantee: Valeria Cristina Sandrim
• Support Opportunities: Regular Research Grants