| Full text | |
| Author(s): |
Freitas, Ana C.
[1]
;
Stafuzza, Nedenia B.
[2]
;
Barbero, Marina M. D.
[1]
;
Santos, Daniel J. A.
[1]
;
Fortes, Marina R. S.
[3]
;
Tonhati, Humberto
[1]
Total Authors: 6
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci FCAV, Dept Anim Sci, BR-14884900 Jaboticabal, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci FCAV, Dept Exact Sci, BR-14884900 Jaboticabal, SP - Brazil
[3] Univ Queensland, Sch Chem & Mol Biosci, St Lucia Campus, Brisbane, Qld 4067 - Australia
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | TROPICAL ANIMAL HEALTH AND PRODUCTION; v. 52, n. 1, p. 415-423, JAN 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Animal breeding programs have used molecular genetic tools as an auxiliary method to identify and select animals with superior genetic merit for milk production and milk quality traits as well as disease resistance. Genes of the major histocompatibility complex (MHC) are important molecular markers for disease resistance that could be applied for genetic selection. The aim of this study was to identify single nucleotide polymorphisms (SNPs) and haplotypes in DRB2, DRB3, DMA, and DMB genes in Murrah breed and to analyze the association between molecular markers and milk, fat, protein and mozzarella production, fat and protein percentage, and somatic cell count. Two hundred DNA samples from Murrah buffaloes were used. The target regions of candidate genes were amplified by polymerase chain reaction (PCR) followed by sequencing and identification of polymorphisms. Allele and genotype frequencies, as well as linkage disequilibrium between SNPs, were calculated. Genotypes were used in association analyses with milk production and quality traits. Except for the DMA gene, identified as monomorphic, the other genes presented several polymorphisms. The DMB, DRB2, and DRB3 genes presented two, six, and seven SNPs, respectively. Fifty-seven haplotype blocks were constructed from 15 SNPs identified, which was used in association analyses. All the studied traits had at least one associated haplotype. In conclusion, it is suggested that the haplotypes found herein can be associated with important traits related to milk production and quality. (AU) | |
| FAPESP's process: | 15/12396-1 - IMPUTATION OF MARKER GENOTYPES IN BUFFALO GENOME AND ITS IMPACT ON GENOMIC EVALUATION OF MILK YIELD AND CONTENT |
| Grantee: | Daniel Jordan de Abreu Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/25309-7 - Polymorphims and expression of the immune system genes associated with mastitis in dairy buffaloes |
| Grantee: | Humberto Tonhati |
| Support Opportunities: | Regular Research Grants |