Polymorphims and expression of the immune system genes associated with mastitis in dairy buffaloes

• Grant number: 14/25309-7
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2015 - February 28, 2017
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Humberto Tonhati
• Grantee: Humberto Tonhati
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

In Brazil, buffaloes stand out in dairy farming due to profit generated. However, economic losses due to changes in the production and composition of milk, making it less suitable for consumption and processing, are caused by mastitis, an infection of the mammary gland caused by a reaction to metabolic and physiological changes, trauma, or microorganism's invasion. Thus, the objective of this study is to evaluate in buffaloes with mastitis the gene expression level of genes lactoferrin, transferrin, ²-defensins and interleukin, and identify, polymorphisms in genes DRB2, DRB3, DMA and DMB. For the analysis of gene expression, samples of milk from animals diagnosed with mastitis and free of infection animals will be collected for RNA extraction, cDNA synthesis and validation expression profiling by qRT-PCR. The study of the expression of these genes related to active immune system in mammary gland can become an important tool for the developing of strategies aimed at preserving the health of the udder. For the study of polymorphisms, the samples of dander will be collected for DNA extraction, PCR and sequencing. MHC complex genes are related to the innate and adaptive system of the animal, so the identification of polymorphisms in these genes may provide relevant information about the allelic and genotypic variability of a given population, and provide markers for susceptibility / resistance to disease economically harmful to livestock, such as mastitis. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA STELLA, ALINE APARECIDA; SIMIELLI FONSECA, LARISSA FERNANDA; JOVINO GIMENEZ, DANIELE FERNANDA; TANAMATI, FERNANDA; DO NASCIMENTO, ANDRE VIEIRA; CARDOSO, DIERCLES FRANCISCO; TONHATI, HUMBERTO. Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis. MOLECULAR AND CELLULAR PROBES, v. 41, p. 39-42, OCT 2018. (14/25309-7)

ROLDAN-MONTES, VALENTINA; CARDOSO, DIERCLES FRANCISCO; HURTADO-LUGO, NAUDIN ALEJANDRO; DO NASCIMENTO, ANDRE VIEIRA; SANTOS, DANIEL JORDAN DE ABREU; SCALEZ, DAIANE CRISTINA BECKER; DE FREITAS, ANA CLAUDIA; HERRERA, ANA CRISTINA; ALBUQUERQUE, LUCIA GALVAO; DE CAMARGO, GREGORIO MIGUEL FERREIRA; et al. Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis). FRONTIERS IN VETERINARY SCIENCE, v. 7, NOV 5 2020. (14/25309-7)

TANAMATI, FERNANDA; TAYLOR, JEREMY F.; BEHURA, SUSANTA K.; SANTOS, DANIEL J. A.; STAFUZZA, NEDENIA B.; ANDRADE, WILLIAN B. F.; GASPARINO, ELIANE; TONHATI, HUMBERTO. Short communication: Characterization of the milk protein expression profiles in dairy buffaloes with and without subclinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 103, n. 3, p. 2677-2684, MAR 2020. (16/10526-8, 14/19321-4, 14/25309-7)

TANAMATI, F.; STAFUZZA, N. B.; GIMENEZ, D. F. J.; STELLA, A. A. S.; SANTOS, D. J. A.; FERRO, M. I. T.; ALBUQUERQUE, L. G.; GASPARINO, E.; TONHATI, H.. Differential expression of immune response genes associated with subclinical mastitis in dairy buffaloes. ANIMAL, v. 13, n. 8, p. 1651-1657, AUG 2019. (14/19321-4, 14/25309-7)

FREITAS, ANA C.; STAFUZZA, NEDENIA B.; BARBERO, MARINA M. D.; SANTOS, DANIEL J. A.; FORTES, MARINA R. S.; TONHATI, HUMBERTO. Polymorphisms in major histocompatibility complex genes and its associations with milk quality in Murrah buffaloes. TROPICAL ANIMAL HEALTH AND PRODUCTION, v. 52, n. 1, p. 415-423, JAN 2020. (15/12396-1, 14/25309-7)