NGAL and SMAD1 gene expression in the early detection of diabetic nephropathy by liquid biopsy

Author(s):Show less -

Veiga, Glaucia ^[1] ; Alves, Beatriz ^[1] ; Perez, Matheus ^[1] ; Alcantara, Luiz Vinicius ^[1] ; Raimundo, Joyce ^[1] ; Zambrano, Lysien ^[2] ; Encina, Jessica ^[1] ; Pereira, Edimar Cristiano ^[3] ; Bacci, Marcelo ^[1] ; Murad, Neif ^[1] ; Fonseca, Fernando ^{[1, 3]}

Affiliation

^[1] Ctr Univ Saude ABC, Fac Med ABC, Santo Andre, SP - Brazil

^[2] Univ Nacl Autonoma Honduras, Tegucigalpa, Francisco Moraz - Honduras

^[3] Univ Fed Sao Paulo, Pharmaceut Sci Dept, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Clinical Pathology; v. 73, n. 11, p. 713-721, NOV 2020.
• Web of Science Citations: 0

Abstract

Introduction Diabetic nephropathy (DN) is a disease that progresses with the slow and progressive decline of the glomerular filtration rate (GFR); the installation of this pathology is silent and one of the major causes of death in patients with diabetes. Aims To identify new molecular biomarkers for early identification of the onset of DN in patients with type II diabetes mellitus (DM2). We studied the expression profile of the genes; suppressor of mothers against decapentaplegic type 1 (SMAD1), neutrophil gelatinase-associated lipocalin (NGAL) and type IV collagen (COLIV1A) in peripheral blood and urine sediment samples. Methods Ninety volunteers, 51 with DM2 and 39 healthy, were recruited from the Faculdade de Medicina do ABC outpatient clinic. We conducted an interview and collected anthropometric data, as well as blood and urine samples for biochemical evaluation and real-time PCR amplification of the genes of interest. Results Gene expression data: peripheral blood NGAL (DM2 0.09758 +/- 0.1914 vs CTL 0.02293 +/- 0.04578), SMAD1 (blood: DM2 0.01102 +/- 0.04059{*} vs CTL 0.0001317 +/- 0.0003609; urine: DM2 0.7195 +/- 2.344{*} vs CTL 0.09812 +/- 0.4755), there was no significant expression of COLIV1A. These genes demonstrated good sensitivity and specificity in the receiving operating characteristic curve evaluation. Conclusion Our data suggest the potential use of NGAL and SMAD1 gene expression in peripheral blood and urine samples as early biomarkers of DN. (AU)

• FAPESP's process: 18/24808-0 - Evaluation of biomarkers of renal damage and inflammatory profile in elderly diabetic rats
• Grantee: Glaucia Raquel Luciano da Veiga
• Support Opportunities: Regular Research Grants