| Full text | |
| Author(s): |
Teixeira, Aline F.
[1]
;
Cavenague, Maria F.
[1, 2]
;
Kochi, Leandro T.
[1, 2]
;
Fernandes, Luis G.
[1]
;
Souza, Gisele O.
[3]
;
de Souza Filho, Antonio Francisco
[3]
;
Vasconcellos, Silvio A.
[3]
;
Heinemann, Marcos Bryan
[3]
;
Nascimento, Ana L. T. O.
[1]
Total Authors: 9
|
| Affiliation: | [1] Inst Butantan, Lab Desenvolvimento Vacinas, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Lab Zoonoses Bacterianas, Dept Med Vet Prevent & Saude Anim VPS, Fac Med Vet & Zootecnia, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | FRONTIERS IN IMMUNOLOGY; v. 11, OCT 30 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Leptospirosis is a zoonotic disease of worldwide distribution, affecting both humans and animals. The development of an effective vaccine against leptospirosis has long been pursued but without success. Humans are contaminated after direct contact with the urine of infected animals or indirectly by contaminated water or soil. The vaccines available consist of inactivated whole-bacterial cells, and the active immunoprotective antigen is the lipopolysaccharide moiety, which is also the basis for serovar classification. However, these vaccines are short-lasting, and protection is only against serovars contained in the preparation. The search for prevalent antigens, present in pathogenic species of Leptospira, represents the most cost-effective strategy for prevention of leptospirosis. Thus, the identification of these antigens is a priority. In this study, we examined the immunoprotective effect of eight leptospiral recombinant proteins using hamster as the challenge model. Animals received subcutaneously two doses of vaccine containing 50 mu g of each recombinant protein adsorbed on alum adjuvant. Two weeks after the booster, animals were challenged with virulent leptospires and monitored for 21 days. All proteins were able to induce a specific immune response, although significant protective effects on survival rate were observed only for the proteins Lsa14, rLIC13259, and rLIC11711. Of these, only rLIC13259 and rLIC11711 were found to be highly prospective in promoting renal clearance. The sterilizing potential of both proteins will be further investigated to elucidate the immunoprotective mechanisms involved in leptospirosis control. These are the first proteins involved with human complement components with the capacity to protect against virulent challenge and to eliminate the bacteria from the host. (AU) | |
| FAPESP's process: | 14/50981-0 - Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis |
| Grantee: | Ana Lucia Tabet Oller do Nascimento |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/06731-8 - Application of CRISPR-interference for elucidating leptospirosis pathogenesis and development of novel strategies for knockout mutant's obtainment |
| Grantee: | Luis Guilherme Virgílio Fernandes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/09652-4 - Evaluation of the interaction of Leptospira interrogans proteins with host components by constructing recombinant Leptospira biflexa |
| Grantee: | Leandro Toshio Kochi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/08131-0 - Heterologous expression of the gene LIC13259 in the saprophyte Leptospira biflexa and their acquisition capacity to evade the human immune system |
| Grantee: | Maria Fernanda Cavenague Pereira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/11541-0 - Evaluation of the vaccine potential of antigenic fractions obtained in Leptospira interrogans serovar Copenhageni using hamsters as an animal model |
| Grantee: | Aline Florencio Teixeira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |