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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Uncovering the genomic potential of the Amazon River microbiome to degrade rainforest organic matter

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Author(s):
Santos-Junior, Celio Dias [1, 2] ; Sarmento, Hugo [3] ; de Miranda, Fernando Pellon [4] ; Henrique-Silva, Flavio [1] ; Logares, Ramiro [5]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Carlos UFSCar, Mol Biol Lab, Dept Genet & Evolut DGE, Rod Washington Luis KM 235 Monjolinho, BR-13565905 Sao Carlos, SP - Brazil
[2] Fudan Univ, Inst Sci & Technol Brain Inspired Intelligence IS, Handan Rd 220, Shanghai 200433 - Peoples R China
[3] Univ Fed Sao Carlos UFSCar, Lab Microbial Proc & Biodivers, Dept Hydrobiol DHB, Via Washington Luis KM 235 Monjolinho, BR-13565905 Sao Carlos, SP - Brazil
[4] Petr Brasileiro SA Petrobras, Ctr Pesquisas & Desenvolvimento Leopoldo Americo, Ave Horacio Macedo 950, BR-21941915 Rio De Janeiro, RJ - Brazil
[5] CSIC, Inst Marine Sci ICM, Passeig Maritim Barceloneta 37-49, ES-08003 Barcelona, Catalonia - Spain
Total Affiliations: 5
Document type: Journal article
Source: MICROBIOME; v. 8, n. 1 OCT 30 2020.
Web of Science Citations: 0
Abstract

Background: The Amazon River is one of the largest in the world and receives huge amounts of terrestrial organic matter (TeOM) from the surrounding rainforest. Despite this TeOM is typically recalcitrant (i.e. resistant to degradation), only a small fraction of it reaches the ocean, pointing to a substantial TeOM degradation by the river microbiome. Yet, microbial genes involved in TeOM degradation in the Amazon River were barely known. Here, we examined the Amazon River microbiome by analysing 106 metagenomes from 30 sampling points distributed along the river. ResultsL We constructed the Amazon River basin Microbial non-redundant Gene Catalogue (AMnrGC) that includes similar to 3.7 million non-redundant genes, affiliating mostly to bacteria. We found that the Amazon River microbiome contains a substantial gene-novelty compared to other relevant known environments (rivers and rainforest soil). Genes encoding for proteins potentially involved in lignin degradation pathways were correlated to tripartite tricarboxylates transporters and hemicellulose degradation machinery, pointing to a possible priming effect. Based on this, we propose a model on how the degradation of recalcitrant TeOM could be modulated by labile compounds in the Amazon River waters. Our results also suggest changes of the microbial community and its genomic potential along the river course. Conclusions: Our work contributes to expand significantly our comprehension of the world's largest river microbiome and its potential metabolism related to TeOM degradation. Furthermore, the produced gene catalogue (AMnrGC) represents an important resource for future research in tropical rivers. (AU)

FAPESP's process: 14/14139-3 - Microbial processes and biodiversity in aquatic ecosystems
Grantee:Hugo Miguel Preto de Morais Sarmento
Support Opportunities: Research Grants - Young Investigators Grants