Tempol improves redox status in mdx dystrophic diaphragm muscle

Author(s)

Hermes, Tulio de Almeida ^[1] ; Mizobuti, Daniela Sayuri ^[1] ; da Rocha, Guilherme Luiz ^[1] ; da Silva, Heloina Nathallie Mariano ^[1] ; Covatti, Caroline ^[1] ; Pereira, Elaine Cristina Leite ^{[2, 1]} ; Ferretti, Renato ^[3] ; Minatel, Elaine ^[1]

Affiliation

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Brasilia UNB, Fac Ceilandia, Brasilia, DF - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Anat, Botucatu, SP - Brazil

• Document type: Journal article
• Source: International Journal of Experimental Pathology; v. 101, n. 6, p. 289-297, DEC 2020.
• Web of Science Citations: 0

Abstract

Oxidative stress is a critical element in relationship to the pathophysiology of Duchenne muscular dystrophy (DMD). In the mice the diaphragm (DIA) is most resembles the dystrophic human pathology. In this study we have evaluated the consequences of a synthetic antioxidant (tempol) on oxidative stress parameters in the DIA muscle of mdx mice. The mdx mice were separated into two groups: mdx, the control group receiving intraperitoneal (i.p.) injections of saline solution (100 mu L), and mdxT, the treated group receiving i.p. injections of tempol (100 mg/kg). The tempol-treated group showed reduced oxidative stress markers, decreasing the dihydroethidium reaction (DHE) area; autofluorescent lipofuscin granules; and 4-hydroxynonenal (4-HNE)-protein adduct levels. DIA muscle of mdx mice. At the same time, the manganese-superoxide dismutase 2 (SOD2) levels were increased in the tempol-treated group. In addition, the tempol-treated group showed reduced levels of glutathione-disulphide reductase (GSR), glutathione peroxidase 1 (GPx1) and catalase (CAT) in immunoblots. The tempol-treated group has also shown lower relative gene expression of SOD1, CAT and GPx than the non-treated group. Our data demonstrated that tempol treatment reduced oxidant parameters and increased anti-oxidant SOD2 levels in the DIA muscle of mdx mice, which may contribute to the normalization of the redox homeostasis of dystrophic muscles. (AU)

• FAPESP's process: 17/01638-0 - TEMPOL: POTENTIAL ANTIOXIDANT THERAPY FOR DYSTROPHIC MUSCULAR FIBERS
• Grantee: Elaine Minatel
• Support Opportunities: Regular Research Grants