| Full text | |
| Author(s): |
Dantas Breda, Leandro Carvalho
[1, 2]
;
de Souza Breda, Cristiane Naffah
[1]
;
Fogaca de Almeida, Jose Roberto
[2]
;
Monteiro Paulo, Larissa Neves
[2]
;
Jannuzzi, Grasielle Pereira
[2]
;
Menezes, Isabela de Godoy
[2]
;
Albuquerque, Renata Chaves
[2]
;
Saraiva Camara, Niels Olsen
[1]
;
Ferreira, Karen Spadari
[3]
;
de Almeida, Sandro Rogerio
[1, 2]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Inst Ciancias Nat, Dept Ciencias Farmaceut, Diadema - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | FRONTIERS IN IMMUNOLOGY; v. 11, OCT 21 2020. |
| Web of Science Citations: | 2 |
| Abstract | |
Chromoblastomycosis is a chronic and progressive subcutaneous mycosis caused mainly by the fungus Fonsecaea pedrosoi. The infection is characterized by erythematous papules and histological sections demonstrating an external layer of fibrous tissue and an internal layer of thick granulomatous inflammatory tissue containing mainly macrophages and neutrophils. Several groups are studying the roles of the innate and adaptive immune systems in F. pedrosoi infection; however, few studies have focused on the role of neutrophils in this infection. In the current study, we verify the importance of murine neutrophils in the killing of F. pedrosoi conidia and hyphae. We demonstrate that phagocytosis and reactive oxygen species during infection with conidia are TLR-2- and TLR-4-dependent and are essential for conidial killing. Meanwhile, hyphal killing occurs by NET formation in a TLR-2-, TLR-4-, and ROS-independent manner. In vivo experiments show that TLR-2 and TLR-4 are also important in chromoblastomycosis infection. TLR-2KO and TLR-4KO animals had lower levels of CCL3 and CXCL1 chemokines and impaired neutrophil migration to the infected site. These animals also had higher fungal loads during infection with F. pedrosoi conidia, confirming that TLR-2 and TLR-4 are essential receptors for F. pedrosoi recognition and immune system activation. Therefore, this study demonstrates for the first time that neutrophil activation during F. pedrosoi is conidial or hyphal-specific with TLR-2 and TLR-4 being essential during conidial infection but unnecessary for hyphal killing by neutrophils. (AU) | |
| FAPESP's process: | 12/18598-7 - Role of pattern recognition receptors (PRRs) in the activation of macrophages in cutaneous fungal infections caused by Fonsecaea pedrosoi, Sporothrix schenckii and Trichophyton rubrum |
| Grantee: | Sandro Rogerio de Almeida |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/11146-9 - The role of neutrophils and Toll Like Receptors 2 and 4 in experimental chromoblastomycosis by F. pedrosoi |
| Grantee: | Leandro Carvalho Dantas Breda |
| Support Opportunities: | Scholarships in Brazil - Doctorate |