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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dataset on recombinant expression of an ancient chitinase gene from different species of Leishmania parasites in bacteria and in Spodoptera frugiperda cells using baculovirus

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Author(s):
Cabral, Aline Diniz [1] ; Garcia, Felipe Baena [1] ; Suzuki, Rodrigo Buzinaro [1, 2, 3] ; Gois Filho, Tanil Lacerda [1] ; da Costa, Renata Torres [1] ; Pereira Vasconcelos, Ligia Marinho [1] ; Santos, Edmar Silva [1] ; Speranca, Marcia Aparecida [1]
Total Authors: 8
Affiliation:
[1] Univ Fed ABC, Ctr Nat & Human Sci, Rua Arcturus 03, Bloco Delta, Sala 226, Lab 107, Sao Bernardo Do Campo, SP - Brazil
[2] Marilia Med Sch, Dept Parasitol, BR-17519030 Marilia, SP - Brazil
[3] Univ Marilia, Sch Med, BR-17525902 Marilia, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: DATA IN BRIEF; v. 32, OCT 2020.
Web of Science Citations: 0
Abstract

The data presented here is related to negative results obtained with the recombinant expression of chitinase from four species of Leishmania parasites in two expression systems, performed in order to investigate the molecular characteristics of the Leishmania chitinase and its possible application in leishmaniasis diagnosis. Thus, heterologous Leishmania sp chitinase proteins were expressed in bacteria using the prokaryotic expression vector pET28a and Escherichia coli Mach-T1, and in Spodoptera frugiperda (Sf9) insect cells, using the eukaryotic bac-to-bac expression system (Thermo Fisher Scientific) to produce recombinant baculoviruses to infect Sf9. Biochemical and cellular analysis of the various recombinant forms of the Leishmania sp chitinase produced in prokaryotic and eukaryotic expression systems were performed through SDS-PAGE and Western blotting. Chitinase produced and purified from bacteria presented low yield and formed inactive aggregates. Heterologous chitinase obtained after infection of Sf9 insect cells with all the four Leishmania species recombinant baculoviruses presented high yield of insoluble proteins. Dot-blot serological tests presented inconclusive results against the recombinant Leishmania sp chitinases produced in both expression systems. The experiments described in this paper can help researchers to avoid errors when choosing a recombinant expression systems to produce Leishmania parasites proteins for biotechnological purposes. (C) 2020 The Author(s). Published by Elsevier Inc. (AU)

FAPESP's process: 13/26096-4 - Heterologous expression of the chitinase enzyme of Leishmania (L.) infantum chagasi, Leishmania (V.) braziliensis and Leishmania (V.) amazonensis: serological diagnosis and comparative molecular study using expression systems of insects cell and bacteria
Grantee:Aline Diniz Cabral
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/14514-4 - Characterization of the chitinase from South American endemic Leishmania species: use in diagnosis in humans, dogs and sandflies.
Grantee:Marcia Aparecida Speranca
Support Opportunities: Regular Research Grants
FAPESP's process: 12/20221-9 - Molecular and serological diagnostic of Leishmania species in clinical samples of patients and dogs from the Midwest of São Paulo, Brazil, presenting visceral leishmaniasis
Grantee:Marcia Aparecida Speranca
Support Opportunities: Regular Research Grants