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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differentially expressed urinary exo-miRs and clinical outcomes in kidney recipients on short-term tacrolimus therapy: a pilot study

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Author(s):
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Costa de Freitas, Renata Caroline [1] ; Bortolin, Raul Hernandes [1] ; Vecchia Genvigir, Fabiana Dalla [1] ; Bonezi, Vivian [1] ; Crespo Hirata, Thiago Dominguez [1] ; Felipe, Claudia Rosso [2] ; Tedesco-Silva, Jr., Helio [2] ; Medina-Pestana, Jose Osmar [2] ; Cerda, Alvaro [3] ; Doi, Sonia Quateli [4] ; Hirata, Mario Hiroyuki [1] ; Crespo Hirata, Rosario Dominguez [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Hosp Rim, Nephrol Div, BR-04038002 Sao Paulo - Brazil
[3] Univ La Frontera, BIOREN, Ctr Excellence Translat Med, Dept Basic Sci, Temuco 4810296 - Chile
[4] Uniformed Serv Univ Hlth Sci, Sch Med, Bethesda, MD 20814 - USA
Total Affiliations: 4
Document type: Journal article
Source: Epigenomics; v. 12, n. 22 DEC 2020.
Web of Science Citations: 0
Abstract

Aim: To analyze the expression of urinary exosome-derived miRNAs (exo-miRs) in kidney recipients on tacrolimus-based therapy. Patients \& methods: Clinical and drug monitoring data were recorded from 23 kidney recipients. Expression of 93 exo-miRs was measured by quantitative PCR array and mRNA targets were explored. Results: 16 exo-miRs were differentially expressed, including marked upregulation of miR-155-5p, and downregulation of miR-223-3p and miR-1228-3p. Expression of miR-155-5p and miR-223-3p correlated with tacrolimus dose (p < 0.05), miR-223-3p with serum creatinine (p < 0.05), and miR-223-3p and miR-1228-3p with blood leukocytes (p < 0.05). 12 miRNAs have predicted targets involved in cell proliferation, apoptosis, stress response, PIK3/AKT/mTOR and TGF-beta signaling pathways. Conclusion: Differentially expressed urinary exo-miRs may be useful markers to monitor tacrolimus therapy and graft function in kidney transplantation. (AU)

FAPESP's process: 16/13118-8 - Pharmacogenomics and epigenomics factors associated with response to immunosuppressive drugs in renal transplant recipients
Grantee:Rosario Dominguez Crespo Hirata
Support type: Regular Research Grants