Differentially expressed urinary exo-miRs and clinical outcomes in kidney recipients on short-term tacrolimus therapy: a pilot study

Author(s):Show less -

Costa de Freitas, Renata Caroline ^[1] ; Bortolin, Raul Hernandes ^[1] ; Vecchia Genvigir, Fabiana Dalla ^[1] ; Bonezi, Vivian ^[1] ; Crespo Hirata, Thiago Dominguez ^[1] ; Felipe, Claudia Rosso ^[2] ; Tedesco-Silva, Jr., Helio ^[2] ; Medina-Pestana, Jose Osmar ^[2] ; Cerda, Alvaro ^[3] ; Doi, Sonia Quateli ^[4] ; Hirata, Mario Hiroyuki ^[1] ; Crespo Hirata, Rosario Dominguez ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Hosp Rim, Nephrol Div, BR-04038002 Sao Paulo - Brazil

^[3] Univ La Frontera, BIOREN, Ctr Excellence Translat Med, Dept Basic Sci, Temuco 4810296 - Chile

^[4] Uniformed Serv Univ Hlth Sci, Sch Med, Bethesda, MD 20814 - USA

• Document type: Journal article
• Source: Epigenomics; v. 12, n. 22 DEC 2020.
• Web of Science Citations: 0

Abstract

Aim: To analyze the expression of urinary exosome-derived miRNAs (exo-miRs) in kidney recipients on tacrolimus-based therapy. Patients \& methods: Clinical and drug monitoring data were recorded from 23 kidney recipients. Expression of 93 exo-miRs was measured by quantitative PCR array and mRNA targets were explored. Results: 16 exo-miRs were differentially expressed, including marked upregulation of miR-155-5p, and downregulation of miR-223-3p and miR-1228-3p. Expression of miR-155-5p and miR-223-3p correlated with tacrolimus dose (p < 0.05), miR-223-3p with serum creatinine (p < 0.05), and miR-223-3p and miR-1228-3p with blood leukocytes (p < 0.05). 12 miRNAs have predicted targets involved in cell proliferation, apoptosis, stress response, PIK3/AKT/mTOR and TGF-beta signaling pathways. Conclusion: Differentially expressed urinary exo-miRs may be useful markers to monitor tacrolimus therapy and graft function in kidney transplantation. (AU)

• FAPESP's process: 16/13118-8 - Pharmacogenomics and epigenomics factors associated with response to immunosuppressive drugs in renal transplant recipients
• Grantee: Rosario Dominguez Crespo Hirata
• Support type: Regular Research Grants