| Full text | |
| Author(s): Show less - |
Hieda, Daniele Seo
[1]
;
da Costa Carvalho, Larissa Anastacio
[1]
;
de Mello, Barbara Vaz
[1]
;
de Oliveira, Erica Aparecida
[1]
;
de Assis, Silvia Romano
[1]
;
Wu, Joanna
[2]
;
Du-Thumm, Laurence
[2]
;
Viana da Silva, Claudia Larissa
[1]
;
Roubicek, Deborah Arnsdorff
[3]
;
Maria-Engler, Silvya Stuchi
[1]
;
de Moraes Barros, Silvia Berlanga
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[2] Colgate Palmol Co, Piscataway, NJ - USA
[3] CETESB Companhia Ambiental Estado Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF INVESTIGATIVE DERMATOLOGY; v. 140, n. 12, p. 2343+, DEC 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Knowing the damage that particulate matter (PM) can cause in skin is important for tightly controlling the release of air pollutants and preventing more serious diseases. This study investigates if such alterations are present in reconstructed human epidermis exposed to coarse air PM. Exposure of reconstructed human epidermis to increasing concentrations (2.2, 8.9, and 17.9 mu g/cm(2)) of standard urban PM over time led to decreased cell viability at 48 hours. The barrier function was shown to be compromised by 24 hours of exposure to high doses (17.9 mu g/cm(2)). Morphological alterations included cytoplasm vacuolization and partial loss of epidermal stratification. Cytokeratin 10, involucrin, loricrin, and filaggrin protein levels were significantly decreased. We confirmed an inflammatory process by IL-1 alpha release and found a significant increase in AQP3 expression. We also demonstrated changes in NOTCH1 and AhR expression of epidermis treated with coarse air PM. The use of hydrogen peroxide altered AQP3 and NOTCH1 expression, and the use of N-acetyl-Lcysteine altered NOTCH1 expression, suggesting that this is a redox-dependent process. These results demonstrate that coarse air PM induces dose-dependent inflammatory response and alterations in protein markers of differentiation and water transport in the epidermis that could ultimately compromise the structural integrity of the skin, promoting or exacerbating various skin diseases. (AU) | |
| FAPESP's process: | 18/14936-1 - Mitochondrial metabolism dictates the heterogeneity and resistance of melanoma |
| Grantee: | Larissa Anastacio da Costa Carvalho |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/19963-1 - AIR POLLUTION AND ATOPIC DERMATITIS |
| Grantee: | Silvia Berlanga de Moraes Barros |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/04926-6 - Melanoma and Chemoresistance: in vitro and in silico models to exploit therapeutic targets |
| Grantee: | Silvya Stuchi Maria-Engler |
| Support Opportunities: | Research Projects - Thematic Grants |