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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intra- and Inter-Host Assessment of Bartonella Diversity with Focus on Non-Hematophagous Bats and Associated Ectoparasites from Brazil

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Author(s):
Ikeda, Priscila [1] ; Marinho Torres, Jaire [2] ; Perles, Livia [1] ; Lourenco, Elizabete Captivo [3] ; Herrera, Heitor Miraglia [2] ; de Oliveira, Carina Elisei [2] ; Zacarias Machado, Rosangela [1] ; Andre, Marcos Rogerio [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Julio de Mesquita Filho, Lab Imunoparasitol, Dept Patol Reprod & Saude Unica, BR-14884900 Sao Paulo - Brazil
[2] Univ Catolica Dom Bosco, Lab Biol Parasitaria, Programa Pos Grad Biotecnol, BR-79117900 Campo Grande, MS - Brazil
[3] Univ Estado Rio de Janeiro, Lab Ecol Mamiferos, BR-20550013 Rio De Janeiro - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MICROORGANISMS; v. 8, n. 11 NOV 2020.
Web of Science Citations: 0
Abstract

The relationship among bats, ectoparasites and associated microorganisms is important to investigate how humans can become exposed to zoonotic agents. Even though the diversity of Bartonella spp. in bats and ectoparasites has been previously reported, the occurrence of gltA genotypes within hosts has not been assessed so far. We aimed to investigate the genetic diversity of Bartonella spp. in non-hematophagous bats and associated ectoparasites by assessing cloned gltA Bartonella genotypes in intra- and inter-hosts levels, as well as by using three additional molecular markers. Overall, 13.5% (18/133) bat blood samples, 17.18% bat flies (11/64) and 23.8% (5/21) Macronyssidae mite pools showed to be positive for Bartonella spp. Seventeen positive samples were submitted to gltA-cloning and three clones were sequenced for each sample. We also obtained 11, seven and three sequences for nuoG, rpoB and ftsZ genes, respectively. None were positive for the other target genes. We found at least two genotypes among the three gltA-cloned sequences from each sample, and 13 between all the 51 sequences. Among the nuoG, rpoB and ftsZ sequences we found eight, five and three genotypes, respectively. In the phylogenetic analysis, the sequences were positioned mainly in groups related to Bartonella identified in rodents, bats and bat flies. Herein, we showed the genetic diversity of Bartonella in bat's blood and associated ectoparasites samples at both intra- and inter-host levels. (AU)

FAPESP's process: 17/14124-4 - Detection and molecular characterization of Anaplasmataceae, Bartonellaceae and Mycoplasmaceae agents in bats sampled in a peri-urban region of Central-Western Brazil
Grantee:Priscila Ikeda
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/02753-0 - ISOLATION AND GENOTYPING OF Bartonella spp. IN DOMESTIC AND WILD RESERVOIR MAMMALS IN BRAZIL
Grantee:Marcos Rogério André
Support Opportunities: Regular Research Grants