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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods

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Reimao, Juliana Quero [1] ; Coser, Elizabeth Magiolo [2] ; Lee, Monica Ran [1] ; Coelho, Adriano Cappellazzo [2]
Total Authors: 4
[1] Fac Med Jundiai, Dept Morfol & Patol Basicas, BR-13202550 Jundiai - Brazil
[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Anim, BR-13083862 Campinas - Brazil
Total Affiliations: 2
Document type: Review article
Source: MICROORGANISMS; v. 8, n. 11 NOV 2020.
Web of Science Citations: 0

Leishmaniasis is a neglected tropical disease with two main clinical forms: cutaneous and visceral leishmaniasis. Diagnosis of leishmaniasis is still a challenge, concerning the detection and correct identification of the species of the parasite, mainly in endemic areas where the absence of appropriate resources is still a problem. Most accessible methods for diagnosis, particularly in these areas, do not include the identification of each one of more than 20 species responsible for the disease. Here, we summarize the main methods used for the detection and identification of leishmaniasis that can be performed by demonstration of the parasite in biological samples from the patient through microscopic examination, by in vitro culture or animal inoculation; by molecular methods through the detection of parasite DNA; or by immunological methods through the detection of parasite antigens that may be present in urine or through the detection of specific antibodies against the parasite. Potential new methods that can be applied for laboratory diagnosis of leishmaniasis are also discussed. (AU)

FAPESP's process: 16/21171-6 - Paromomycin for the treatment of Tegumentary Leishmaniasis: investigation in vitro, in vivo and in the identification of molecular markers associated with susceptibility and resistance
Grantee:Adriano Cappellazzo Coelho
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 18/18954-4 - Drug repurposing: evaluation of activity, selectivity and mechanism of action of new drug candidates against Toxoplasma gondii
Grantee:Juliana Quero Reimão Dalla Zanna
Support type: Research Grants - Young Investigators Grants