| Full text | |
| Author(s): Show less - |
Dos Santos, Ancely F.
[1]
;
Inague, Alex
[1]
;
Arini, Gabriel S.
[1]
;
Terra, Leticia F.
[1]
;
Wailemann, Rosangela A. M.
[1]
;
Pimentel, Andre C.
[1]
;
Yoshinaga, Marcos Y.
[1]
;
Silva, Ricardo R.
[2]
;
Severino, Divinomar
[1]
;
de Almeida, Daria Raquel Q.
[1]
;
Gomes, Vinicius M.
[1]
;
Bruni-Cardoso, Alexandre
[1]
;
Terra, Walter R.
[1]
;
Miyamoto, Sayuri
[1]
;
Baptista, Mauricio S.
[1]
;
Labriola, Leticia
[1]
Total Authors: 16
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | CELL DEATH & DISEASE; v. 11, n. 12 DEC 14 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Lack of effective treatments for aggressive breast cancer is still a major global health problem. We have previously reported that photodynamic therapy using methylene blue as photosensitizer (MB-PDT) massively kills metastatic human breast cancer, marginally affecting healthy cells. In this study, we aimed to unveil the molecular mechanisms behind MB-PDT effectiveness and specificity towards tumor cells. Through lipidomics and biochemical approaches, we demonstrated that MB-PDT efficiency and specificity rely on polyunsaturated fatty acid-enriched membranes and on the better capacity to deal with photo-oxidative damage displayed by non-tumorigenic cells. We found out that, in tumorigenic cells, lysosome membrane permeabilization is accompanied by ferroptosis and/or necroptosis. Our results also pointed at a cross-talk between lysosome-dependent cell death (LDCD) and necroptosis induction after photo-oxidation, and contributed to broaden the understanding of MB-PDT-induced mechanisms and specificity in breast cancer cells. Therefore, we demonstrated that efficient approaches could be designed on the basis of lipid composition and metabolic features for hard-to-treat cancers. The results further reinforce MB-PDT as a therapeutic strategy for highly aggressive human breast cancer cells. (AU) | |
| FAPESP's process: | 19/09517-2 - Evaluation of the relationship between energy fluence variation and cell death pathways induced by photodynamic therapy with methylene blue in breast tumors |
| Grantee: | Gabriel Santos Arini |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 17/13804-1 - Mechanisms of detoxification and repair of oxidized biological membranes involving the action of the enzyme peroxiredoxin 6 |
| Grantee: | Alex Inague |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 19/05026-4 - Development of a computational platform extensible and modular for analysis of metabolomics and metagenomics experiments: Innovating with the discovery of new enzymatic activities and derived natural products of interest |
| Grantee: | Ricardo Roberto da Silva |
| Support Opportunities: | Scholarships in Brazil - BIOTA - Young Researchers |
| FAPESP's process: | 16/04676-7 - Photodynamic therapy to treat breast cancer: proof of concept pre-clinical studies |
| Grantee: | Ancély Ferreira dos Santos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/23914-9 - Exploring the immunogenic potential of photodynamic therapy |
| Grantee: | Ancély Ferreira dos Santos |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 17/18922-2 - Development of a computing platform extensible and modular for metabolomics and metagenomics analysis: innovation with the discovery of new enzymatic activities and natural products of pharmaceutical interest derived |
| Grantee: | Ricardo Roberto da Silva |
| Support Opportunities: | BIOTA-FAPESP Program - Young Investigators Grants |
| FAPESP's process: | 17/03618-6 - Unveiling HSPB1's role in prolactin-induced modulation of the unfolded protein response in type 1 Diabetes models |
| Grantee: | Leticia Labriola |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/02654-3 - Multi-User Equipment approved in grant 2014/10492-0 fluorescence microscope |
| Grantee: | Alexandre Bruni Cardoso |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |