Photobiomodulation Therapy Modulates Muscle Gene Expression and Improves Performance of Rats Subjected to a Chronic Resistance Exercise Protocol

Author(s):Show less -

Macedo, Michel Monteiro ^[1] ; Mafra, Fernando Francisco Pazello ^[1] ; Teixeira, Carla de Brito ^[1] ; Torres-Silva, Romildo ^[1] ; Santos Francisco, Raphael Peres dos ^[1] ; Gattai, Pedro Paulo ^{[2, 3]} ; Boim, Mirian Aparecida ^[4] ; Bjordal, Jan Magnus ^[5] ; Nascimento, Fabio Dupart ^[1] ; Leonardo, Patricia Sardinha ^[6] ; Stamborowski, Sadi S. F. ^[6] ; Lopes-Martins, Rodrigo alvaro Brandao ^[6]

Affiliation

^[1] Univ Mogi Cruzes UMC, Postgrad Program Biomed Engn, Technol Res Ctr NPT, Sao Paulo - Brazil

^[2] Brazilian Sch Chinese Med EBRAMEC, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Lab Microscop Anat & Dev, Sao Paulo - Brazil

^[4] Fed Univ Sao Paulo UNIFESP, Dept Nephrol, Sao Paulo - Brazil

^[5] Univ Bergen, Dept Global Publ Hlth & Primary Care, Physiotherapy Res Grp, Bergen - Norway

^[6] Univ Vale Paraiba UNIVAP, Inst Res & Dev, Lab Biophoton & Expt Therapeut, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY; v. 38, n. 12, p. 713-719, DEC 1 2020.
• Web of Science Citations: 0

Abstract

Objective:In professional sports activities, the search for increased performance is constant. Electrophysical agents, including photobiomodulation (PBM), have been used in the sports context to accelerate postworkout recovery, prevent injuries, and even to improve performance. This study aims to investigate the effects of infrared laser (904 nm) on skeletal muscle gene expression of performance-related proteins of rats submitted to a chronic resistance training protocol. Materials and methods:Male Wistar rats (n = 40), weighing +/- 300 g were divided into four groups: sedentary control (CT,n = 10); irradiated control (CTL,n = 10); exercised not irradiated (EX,n = 10); exercised irradiated (EXL,n = 10). To assess the performance, the maximum carrying test was adapted and applied 72 h prior the training and 72 h after the last exercise session. The vertical weight climbing protocol was adapted for resistance training 3 x per week with 48 h interval between each session: first week adaptation, second week 25% of body weight (BW), third week 50% BW, fourth week 75% BW, and fifth week 100% BW. Animals were irradiated before exercise on hind paws 50 sec each, with infrared laser 904 nm 5 days per week, during 4 weeks, 9 J per leg in a total of 18 J energy per day. Results:The EXL performed more climbing (7.1 +/- 0.91) compared to EX (4.4 +/- 0.63). PBM promoted increased expression of lactate dehydrogenase enzyme, mammalian target of rapamycin protein, and androgen receptor (p < 0.05) but not the myosin heavy chain (p = 0.43). Conclusions:PBM therapy increases the expression of performance-related muscle mass gain genes besides improving the resistance training performance. (AU)

• FAPESP's process: 15/06502-3 - Analysis and Modelling of Mechanical Behavior of Mechanicaly-lesioned Tendons treated with Low Level Laser Therapy and/or Cryotherapy
• Grantee: Rodrigo Alvaro Brandão Lopes Martins
• Support Opportunities: Regular Research Grants